兔肾近端小管细胞的培养  

THE CULTURE OF PROXIMAL TUBULE CELLS OF RABBIT KIDNEY

作　　者：邵佑之[1] 

机构地区：[1]锦州医学院组织学胚胎学教研室,锦州121004

出　　处：《解剖学报》1991年第3期297-300,T002,共5页Acta Anatomica Sinica

摘　　要：本文将percoll分离的兔肾近端小管细胞,应用微细胞培养插盘,在Ham F 12和Dulbecco's modified Eagle混合培养液中进行双室培养。应用充氧的培养液培养3周后,获得一层完整的近端小管细胞。透射电镜观察表明,细胞具有近端小管细胞的特点,如微绒毛的出现、较丰富的线粒体、吞饮小泡、溶酶体以及不规则的质膜内褶。^(14)C标记的葡萄糖吸收试验,以及“^(14)CO_2释放的测定,说明培养出的一层近端小管细胞重吸收功能完好,代谢旺盛。本文并对该方法进行了较详细的介绍和分析。It is a new approach to investigate the ultrastructure, function and metabolism of each segment of uriniferous tubule using dual environment culture chambers (DECC). In the present study the DECC culture of proximal tubule cells was undertaken with millcell-HA culture insert in the mixed medium of Ham's F12 and Dulbecco's modified Eagle's medium.A monolayer of proximal tubule cells was obtained in the medium mixed by constant bubling of oxygen at 3 weeks after seeding. Transmission electron microscopy revealed the cuboidal cells with microvilli, abundant mitochondria, vacuoles, lysosomes, and irregular plasma membrane infoldings. Also, the monolayer exhibited active metabolism from the release of ^(14)C-I-carbon dioxide and absorption ability of ^(14)C-I-glucose across the cultured monolayer. In addition, the culture procedure has been introduced in detail.

关 键 词：肾 近端小管 细胞培养 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]