水溶性卟啉试剂的合成及其在人发中痕量铜光度法测定中的应用  

Synthesis of water-soluble porphyrin and spectrophotometric study on the determination of trace copper in human hair

作　　者：汤福隆[1] 陈秀华[1] 万林 束勇挥 

机构地区：[1]杭州大学化学系

出　　处：《化学试剂》1989年第3期134-136,共3页Chemical Reagents

基　　金：中国科学院科学基金资助课题

摘　　要：5,10,15,20-四(2-羟基-5-磺酸基苯)卟吩是一新的水溶性卟啉显色剂,简称 T(2-H-5-SP)P。讨论了试剂的合成及其与 Cu^(2+)的显色反应。与 Cu^(2+)反应的最适 pH4.0,不需加辅助络合剂,只要在沸水浴中加热两分钟即可,配合物至少稳定6小时。试剂的质子化形式及其与 Cu^(2+)配合物的最大吸收波长分别于434和412nm。工作曲线的回归方程 A=6.50×10^(-3)+0.132C,相关系数 r=0.999,铜的含量于0～3.0μg/25ml 范围符合比尔定律。配合物的表观摩尔吸光系数ε=2.2×10~5。可用于人发中痕量铜的测定,所得结果与原子吸收光度法很一致。A new water-soluble porphyrin,5,10,15,20-tetra(2-hydroxy-5-sulphophenyl)por-phine[T(2-H-5-SP)P]has been synthesizedand its color reaction with Cu(Ⅱ)was stud-ied.The complex is formed by heating at 100℃for 2 min.in a water-bath and is stable forat least 6 hr.The Soret band of the complexlies at 412nm,which was well separated fromthat of the diacid form of T(2-H-5-SP)P(434nm)below pH 1.The apparent molar absor-ptivity of the complex is 2.2×10~3/·mol^(-1).cm^(-1).Beer's law is obeyed for Cu^(2+) in therange of 0～3μg/25ml.This method can be usedfor the determination of trace copper(Ⅱ)inhuman hair,the result agreed favorably witkthat obtained by AAS.

关 键 词：毛发 铜 光度法 卟啉 试剂 水溶性 

分 类 号：TQ421.3[化学工程]