HIV感染和AIDS患者T淋巴细胞免疫病理改变的研究  

T-lymphocyte immune in HIV-infected people and AIDS patients in China

作　　者：李太生[1] 邱志峰[1] 王爱霞[1] 盛瑞媛[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院感染内科,100730

出　　处：《中华医学杂志》2002年第20期1391-1395,共5页National Medical Journal of China

基　　金：中法先进研究计划合作项目 (PRAB 0 0 0 1)

摘　　要：目的　探讨我国HIV感染和AIDS患者T淋巴细胞的免疫病理改变情况及其临床意义。方法　收集 13例HIV感染患者 (HIV组 )、2 7例AIDS患者 (AIDS组 )和 5 1名健康献血员 (正常对照组 )的抗凝血 ,用人T淋巴细胞亚群的特异性荧光抗体标记 ,通过流式细胞仪检测外周血CD4 + T淋巴细胞 (T4细胞 )、CD8+ T淋巴细胞 (T8细胞 )及其亚群 ,并检测患者血浆HIV载量。结果　正常对照组、HIV组和AIDS组外周血T4细胞计数 (× 10 6个 /L)分别为 84 9± 2 88,4 37± 184 ,5 0± 5 1,组间比较P <0 0 1;T8细胞计数 (× 10 6个 /L)分别为 5 79± 175 ,10 31± 345 ,5 35± 338,HIV组明显高于正常对照组(P <0 0 5 ) ;T4纯真细胞 (CD4 + CD4 5RA+ CD6 2L+ )亚群比例分别为 4 3 0 %± 11 4 % ,4 4 2 %± 12 8% ,2 4 8%± 15 5 % ,AIDS组明显为低 (P <0 0 5 ) ;T4纯真细胞计数 (× 10 6个 /L)分别为 36 8± 16 2 ,185±134,18± 2 0 ,组间比较P <0 0 1;T4功能细胞 (CD4 + CD2 8+ )亚群比例分别为 93 1%± 8 1% ,6 2 6 %±2 8 2 % ,5 6 9%± 2 6 4 % ,组间比较P <0 0 1;T4和T8激活细胞 (CD4 + HLA DR+ ,CD8+ HLA DR+ ,CD8+CD38+ )亚群比例均表现为AIDS组 >HIV组 >正常对照组 (P <0 0 5 ) ;T4和T8凋亡细胞 (CD4 +CD95 + 。Objective To study the T lymphocyte immune alterations and its clinical significance in HIV infected persons and AIDS patients in China Methods Peripheral blood samples were collected from 13 HIV infected persons, 27 AIDS patients, and 51 healthy blood donors (controls) and then labeled with monoclonal antibodies Flow cytometry was used to detect the count of CD4 + T lymphocytes, CD8 + T lymphocytes , and its different subsets including the naive cell subset (CD4 + CD45RA + CD62L +), activated cell subsets(CD4 + HLA DR +,CD8 + HLA DR +,CD8 +CD38 +), functional cell subsets (CD4 +CD28 +), and apoptotic cell subsets (CD4 +CD95 +,CD8 + CD95 +) The plasma HIV loading was also detected Results The CD4 + cell count was (849±288)×10 6/L in the control group,significantly higher than that in HIV infected person group [(437±184)×10 6/L, P <0 01], and that in AIDS patient group [(50±51)×10 6/L, P <0 01] The CD8 + cell count was (579±175)×10 6/L in the control group,significantly lower than that in HIV infected person group [(1 031±345)×10 6/L, P <0 05],and not significantly different from that in AIDS patient group (535±338)×10 6/L, with a significant difference between the HIV infected person group and AIDS patient group( P <0 05) The percentage of CD4 + CD45RA + CD62L + were 43 0%±11 4% in the control group, not significantly different from that in HIV infected person group (44 2%±12 8%), but significantly higher than that in AIDS patient group (24 8%±15 5%, P <0 05) The CD4 + CD45RA + CD62L + cell count was (368±162)×10 6/L in the control group,significantly higher than those in HIV infected person group [(185±134)×10 6/L] and in AIDS patient group [(18±20)×10 6/L, both P <0 01] with a significant difference between the HIV infected person group and AIDS patient group ( P <0 01) The percentage of CD4 +CD28 + was 93 1%±8 1% in the control group,significantly higher than those in HIV infected person

关 键 词：HIV感染 AIDS T淋巴细胞 免疫病理 改变 

分 类 号：R512.91[医药卫生—内科学]