反相高效液相色谱法测定龙胆泻肝丸中黄芩苷的含量  

Determination of baicaline in longdanxiegan pill with reversed-phase HPLC

作　　者：贾善学 李锦绣[2] 赵卫 张军华 

机构地区：[1]山东省滨州市药品检验所,滨州256618 [2]山东省滨州市人民医院,滨州256617

出　　处：《中国新药杂志》2002年第10期782-783,共2页Chinese Journal of New Drugs

摘　　要：目的 :采用反相高效液相色谱法测定龙胆泻肝丸中黄芩苷的含量。方法 :色谱柱为SpherisorbC1 8柱 ,流动相为甲醇 水 醋酸 (45∶55∶1 ) ,UV检测波长为 2 74nm。结果 :黄芩苷峰与其他组分峰的分离度为 3 .0 ,理论塔板数以黄芩苷峰计算为 2 3 32 0 ;方法的平均加样回收率为 98.6 % (n =5) ;黄芩苷在 0 .2 5～ 2 .5μg范围内 ,浓度与峰面积呈良好的线性关系。结论 :本法测定龙胆泻肝丸中黄芩苷的含量 ,结果准确 。Objective:To determine baicaline in Longdanxiegan pill with RP HPLC.Methods:Baicaline was separated on Spherisorb C 18 column using methanol water acetic acid(45∶55∶1) as a mobile phase and UV detection wavelength at 274nm.Results:The resolution was 3.0 and the number of theoretical plates calculated for baicaline peak was 23 320.The average recovery was 98.6%( n =5).There was a good linear relationship between the concentration and peak area value of baicaline in the range of 0.25～2.5μg.Conclusion:The method is highly reproducible and accurate.

关 键 词：反相高效液相色谱法法 龙胆泻肝丸 黄芩苷 含量 测定 中药 

分 类 号：R286.0[医药卫生—中药学]