Observation of the in vitro Transcription of the Mouse (Balb/c) Heart Nuclear DNA Fragments by AFM  

作　　者：袁明秀  Ren Zhong  JIN Rui  Niu Weiran  Zhang Zhihong  Hong Wei  Li Jianwei  

机构地区：[1]College of Life Sciences, Peking University, Beijing 100871, P.R.China [2]The Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, P.R.China

出　　处：《High Technology Letters》2002年第2期23-30,共8页高技术通讯（英文版）

摘　　要：It is observed that the regulation sequences at the two ends of every active gene of the heart nuclear DNA fragments may differentially combine with active regulation factors such as some specific binding proteins by using AFM and other experimental technologies. These active genes form different "gene knots", which are separated by "intervals". Using AFM, occasionally, it is also discovered that during the transcription stage, the heart nuclear DNA fragments consist of 3 4 5 "gene knots" and related "intervals", which form various "gene lineages" respectively by some "permutation and combination". Each gene lineage is likely to form nRNA chain like complexes that are 3 times the quantity of gene knots, and each nRNA chain like complex is connected with both ends of corresponding gene lineage. One gene knot of the DNA fragments participates the formation of different gene lineage and corresponding RNA chain like complexes by different combination. By posttranscriptional modification, they can form nmRNA linear chain like complexes that show the speciality of tissues. The beginnings of transcription units have the same number as gene lineages, and all gene lineages in DNA molecules may transcribe efficiently from corresponding beginnings of transcription unit simultaneously. Our work shows the prospective application of AFM in the research of the diversity of gene lineages formation from gene knots in the transcription stage and the efficiency of gene knots transcription.It is observed that the regulation sequences at the two ends of every active gene of the heart nuclear DNA fragments may differentially combine with active regulation factors such as some specific binding proteins by using AFM and other experimental technologies. These active genes form different 'gene knots', which are separated by 'intervals'. Using AFM, occasionally, it is also discovered that during the transcription stage, the heart nuclear DNA fragments consist of 3 4 5 'gene knots' and related 'intervals', which form various 'gene lineages' respectively by some 'permutation and combination'. Each gene lineage is likely to form nRNA chain like complexes that are 3 times the quantity of gene knots, and each nRNA chain like complex is connected with both ends of corresponding gene lineage. One gene knot of the DNA fragments participates the formation of different gene lineage and corresponding RNA chain like complexes by different combination. By posttranscriptional modification, they can form nmRNA linear chain like complexes that show the speciality of tissues. The beginnings of transcription units have the same number as gene lineages, and all gene lineages in DNA molecules may transcribe efficiently from corresponding beginnings of transcription unit simultaneously. Our work shows the prospective application of AFM in the research of the diversity of gene lineages formation from gene knots in the transcription stage and the efficiency of gene knots transcription.

关 键 词：gene knot transcription group effect gene lineage nRNA chain  like complex nmRNA chain  like complex AFM 

分 类 号：Q523[生物学—生物化学]