病毒感染在小儿肾病发病机制中的作用研究  

A Study on Role of Viruses Infection in Pathogenesis of Nephrotic Syndrome

作　　者：穆莉芳[1] 王峥[1] 纪树萍[1] 

机构地区：[1]华西医科大学附属第二医院儿科

出　　处：《中国医师杂志》2002年第9期956-958,共3页Journal of Chinese Physician

基　　金：国家自然科学基金资助项目 (39870 747)

摘　　要：目的　探讨呼吸道病毒感染与激素敏感型单纯性肾病综合征 (SSSNS)触发的相关性。方法　采用反转录—聚合酶链反应 (RT -PCR)、桥联酶标法 (APAAP)和酶联免疫吸附试验 (ELISA) ,检测 2 6例SSSNS患儿外周血单个核细胞 (PBMC)呼吸道病毒基因、病毒抗原和血清病毒抗体 ,并以正常儿童作对照。结果　⑴SSSNS患儿活动期组PBMC中呼吸道病毒基因、病毒抗原及血清病毒抗体检出率明显高于缓解期组及正常对照组 (P <0 0 5 )。⑵SSSNS患儿病毒检出率与尿蛋白定量密切相关 (P <0 0 5 ) ,2 4h尿蛋白定量大于 5 0mg·kg- 1·d- 1)者 ,病毒检出率高于 2 4h尿蛋白定量小于 5 0mg·kg- 1·d- 1者 ,尿蛋白转阴时 ,体内病毒消失。Objective To explore whether respiratory tract viruses infection was involved in the pathogenesis of steroid sensitive and simple nephrotic syndrome(SSSNS).Methods Expression of respiratory tract viruses gene and antigen in peripheral blood mononuclear cells(PBMC) and production of viruses antibody in sera from 26 children with steroid-sensitive and simple nephrotic syndrome were evaluated by reverse transcription polymerase chain reaction(RT-PCR),APAAP and ELISA.Results In compared in active phase with remission group of SSSNS and normal age-matched controls,the positive rate of viruses of active phase group was significantly increased(P<0 05);The percentage of the detecting viruses of SSSNS had closely correlation with the quantity of urinary protein(P<0 05).Conclusion Onset of SSSNS may be related to the infection of respiratory tract viruse.

关 键 词：发病机制 肾病综合征 病毒基因 儿童 反转录-聚合酶链反应 桥联酶标法 酶联免疫吸附试验 呼吸道病毒感染 

分 类 号：R726.902[医药卫生—儿科]