知母中菝葜皂甙元测定方法探讨  

Determination of Sarsasapogenin in Rhizoma Anemarrhenae

作　　者：伍庆[1] 

机构地区：[1]贵州师范大学分析测试中心,贵州贵阳550001

出　　处：《贵州师范大学学报（自然科学版）》2002年第4期72-74,共3页Journal of Guizhou Normal University：Natural Sciences

摘　　要：建立用高效薄层色谱快速测定知母药材中菝葜皂甙元的方法。药材用苯和20%盐酸共同回流提取,以苯-丙酮(9:1)为展开剂,扫描波长λS=445nm,λR=600nm。结果显示:菝葜皂甙元在0 5～4μg范围内呈良好的线性关系,r=0 9991,平均回收率为98 7%。RSD=2 45%(n=5)。A method was developed for determination of Sarsasapogenin in Rhizoma Anemarrhenae.Method:a mixfare of benzene acetone (9:1)was made as developing solvent: λS=445nm,λR=600nm.Results:The calibration crves were linear between 05～4ug(r=09991).The average recovery was 987%.RSD was 245%(n=5).Conclusion:The method is simpl,rapid,and reproducible

关 键 词：测定方法 菝葜皂甙元 知母 高效薄层色谱法 中药材 含量测定 

分 类 号：R284.1[医药卫生—中药学] Q946.83[医药卫生—中医学]