益气活血开窍复方对缺血再灌注脑组织兴奋性氨基酸及超微结构的影响  

Effects of Yiqi Huoxue Kaiqiao Recipe on excitatory amino acid and ultrastructure in ischemia-reperfusion rats

作　　者：邱根全[1] 石秦东[1] 蔡云[1] 孙喜才[1] 徐羲 

机构地区：[1]西安交通大学第一医院中医科,西安710061 [2]陕西省医学高等专科学校,西安710086

出　　处：《西安交通大学学报（医学版）》2002年第5期489-492,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省卫生厅科研基金资助项目 ( 990 39)

摘　　要：目的　探讨益气活血开窍复方对缺血再灌注脑组织保护作用的机理。方法　以线栓法制备大鼠脑缺血再灌注模型 ,采用全自动氨基酸分析仪、干湿重法、原子吸收分光光度计测量脑组织中兴奋性氨基酸 (EAA)含量、含水量及钙离子 (Ca2 + )含量 ,电子显微镜观察脑组织超微结构改变。结果　中药组谷氨酸 (Glu)含量下降 (P <0 .0 1) ,谷氨酸 /γ 氨基丁酸 (Glu/GABA)趋向于正常比值 ,脑组织含水量及Ca2 + 浓度下降 (P <0 .0 5 )。中药可减轻由于缺血再灌注所导致的细胞超微结构的改变。结论　益气活血开窍方通过抑制EAA释放、Ca2 + 内流而发挥脑组织保护作用。Objective To investigate whether the neuroprotective effects of Yiqi Huoxue Kaiqiao Recipe are related to its inhibition of excitotoxic effect of EAAs. Methods Middle cerebral artery occlusion reperfusion (MCAO/IR) model in rats was made by Longa's method. Automatic amino acid analysis system, dry and wet weight methods, and atomic absorption spectroscopy were employed to measure the excitatory amino acid concentrations, and H 2O and Ca 2+ contents in the brain. The ultrastructure of the neuron was also observed.Results The concentration of glutamate (Glu) in treatment group was significantly lower than that in ischemia/reperfusion group (P< 0.01). The Glu/GABA ratio in treatment group tended to be normal. The content of brain Ca 2+ in treatment group was significantly lower than that in ischemia reperfusion group (P< 0.05). The medicine could mitigate ultrastructural changes of the neuron made by ischemia reperfusion. Conclusion Yiqi Huoxue Kaiqiao Recipe can inhibit the release of EAAs and has a neuroprotective effect.

关 键 词：益气活血开窍复方 中药 大鼠 缺血再灌注 兴奋性氨基酸 超微结构 脑缺血 

分 类 号：R285.5[医药卫生—中药学] R743.31[医药卫生—中医学]