RP-HPLC测定家兔血浆中银杏叶提取物浓度方法研究  

Determination of Extract Ginkgo Biloba in Blood Plasma of Rabbits

作　　者：杨其绛[1] 吴国忠[1] 薛文隽[1] 唐跃年[2] 巫向前[1] 

机构地区：[1]上海第二医科大学卫生技术学院,200092 [2]上海第二医科大学附属新华医院药剂科,200092

出　　处：《医药导报》2002年第11期687-689,共3页Herald of Medicine

基　　金：上海市自然科学研究基金资助项目 (基金编号 :0 1ZB14 0 5 5 )

摘　　要：目的 :建立用反相高效液相色谱 (RP HPLC)测定家兔血浆中银杏叶提取物 (extractginkgobiloba ,EGB)浓度的方法 ,对银杏叶提取物在家兔体内的药物动力学进行研究。方法 :采用ODS Hypersil色谱柱 (10 0mm× 2 .1mm ,5 μm)柱温 2 2℃ ,甲醇 水 磷酸为流动相 (3 2∶67.6∶0 .4) ,流速 1.0mL·min 1 ,紫外检测波长 3 68nm等条件下进行分析测定。结果 :最低检测浓度 1.2 5 μg·mL 1 ,方法平均回收率分别为 98.82 %和 10 1.0 6%精密度为 10 7.80 % ,相对标准差 (RSD) <4%(n =3 ) ,线性范围 2 .5 0～ 5 .0 0 μg·mL 1 (r=0 .9972 ,n =5 )和 6.2 5～ 15 .0 0 μg·mL 1 (r =0 .995 7,n =5 )。结论 :该法具有操作简单、干扰小、准确灵敏等优点。为临床应用EGB提供实验依据。Objective:To establish the method of determining extract ginkgo biloba(EGB) in blood plasma of rabbits by RP HPLC and study the pharmacokinetics of EGB in rabbits. Method:Hypersil column (100 mm×2.1 mm, 5 μm) of the analytical column was ODS. The mobile phase consisted of methanol water phosphate (32∶67.6∶0.4). The flow rate was 1.0 mL·min 1 . The UV detection was at 368 nm. Results:The lower limit of determination was 1.25 μg·mL 1 . The average recovery of the method was 98.82% 107.80%. The related standard deviation ( RSD ) was <4%( n =3). The linear limits of high and low blood concentrations ranged from 2.5 5.00 μg·mL 1 ( r =0.997 2, n =5)and 6.25 15.00 μg·mL 1 ( r =0.995 7, n =5) respectively. Conclusion:The method of determination for EGB in the blood plasma of rabbits by RP HPLC is established in this experiment. It has the advantages of being easy to operate, accurate and sensitive.

关 键 词：RP-HPLC 测定 家兔 血浆 银杏叶提取物 

分 类 号：R284.1[医药卫生—中药学]