The effect of PDGFmRNA and IGFmRNA in the platelet concentrated liquid on ulna fracture healing in rabbit  

作　　者：马维 张英泽 张爱民 张华 郑广德 范志勇 

出　　处：《中国临床康复》2002年第20期3126-3127,I004,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：Objective By hybridization in situ and biomech anical approach of platelet-derive d growth fator mRNA(PDGFmRNA)and in-sulin-like growth factor mRNA(IGFmRNA),we discussed the influence of the platelet concentrated liquid on the healing of rabbit ulna frac-ture.Method We selected 24New Zealand rabbits,divided them into 4groups randomly(blank group,serum-control group,g roup with platelet concentrated liquid and group with b one graft and platelet concentrated liquid),and then made the fracture model on t he middle of ulna which was fixed by finger armor plate.Before t he operation,we drew out 6ml blood fr om femoral artery,performed anti-c oagulation with the Sodium Citrate and centrifugated by low and the followed high speed.We purified the white blood plate and injected it into the fracture position.The rabbits were killed at 1st,2nd,4th and 6th week.Q ualitative analysis by hybridizati on in situ of PDGFmRNAand IGFmRNAand biomechanical measurement on the 6th week sample were made.Result Bone callus could be seen on the radiu s specimen in various degrees when th e rabbits were killed at 1st,2nd,4th and 6th week,particularly i n the last week.The average maximum d estructive load on the fracture tip i s higher to the control,and there is significant difference(P <0.01).Conclusion The local application of platelet co ncentration on the fracture tip can a ccelerate its healing.Objective By hybridization in situ and biomech anical approach of platelet-derive d growth fator mRNA(PDGFmRNA)and in-sulin-like growth factor mRNA(IGFmRNA),we discussed the influence of the platelet concentrated liquid on the healing of rabbit ulna frac-ture.Method We selected 24New Zealand rabbits,divided them into 4groups randomly(blank group,serum-control group,g roup with platelet concentrated liquid and group with b one graft and platelet concentrated liquid),and then made the fracture model on t he middle of ulna which was fixed by finger armor plate.Before t he operation,we drew out 6ml blood fr om femoral artery,performed anti-c oagulation with the Sodium Citrate and centrifugated by low and the followed high speed.We purified the white blood plate and injected it into the fracture position.The rabbits were killed at 1st,2nd,4th and 6th week.Q ualitative analysis by hybridizati on in situ of PDGFmRNAand IGFmRNAand biomechanical measurement on the 6th week sample were made.Result Bone callus could be seen on the radiu s specimen in various degrees when th e rabbits were killed at 1st,2nd,4th and 6th week,particularly i n the last week.The average maximum d estructive load on the fracture tip i s higher to the control,and there is significant difference(P <0.01).Conclusion The local application of platelet co ncentration on the fracture tip can a ccelerate its healing.

关 键 词：尺骨骨折 骨折愈合 血小板浓缩液 血小板源性生长因子 胰岛素样生长因子 

分 类 号：R683.41[医药卫生—骨科学]