地黄对缺氧大鼠心脑肾线粒体呼吸功能的保护作用  

Protective effects of plant of rehmannia root extract on mitochondrial respiratory function of heart, brain and kidney in hypoxia rat

作　　者：汤依群[1] 戴德哉[1] 黄宝[2] 

机构地区：[1]中国药科大学药理研究室,江苏南京210009 [2]杭州娃哈哈集团,浙江杭州310009

出　　处：《中草药》2002年第10期915-917,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的　研究地黄抗缺氧的机制。方法　大鼠处死放置 15 m in,造缺氧模型 ,用氧电极法测定大鼠心室肌、大脑和肾线粒体呼吸功能参数 ,观察地黄浸膏预防给药 2 h对线粒体的保护作用。并以苄普地尔 5 m g/ kg ip给药作阳性对照。结果　地黄浸膏 2 ,4 g/ kg给药 ,对大鼠缺氧心、脑、肾线粒体有明显的保护作用 ,用药后 ST3,RCI值较模型组提高并呈剂量依从关系 ,地黄对肾脏的保护作用相对心、脑强。结论　地黄预防性给药 ,可有效保护心、脑、肾组织线粒体的呼吸产能功能 。Object To observe the protective effects of rehmannia root extract (Reh) on mitochondrial respiratory function in hypoxia rat. Methods Rat was killed rapidly and the body was put at room temperatare for global ischemia of 15 min. Treatment of po Reh 2 and 4 g/kg was started 2 h before hypoxia. The mitochondrial respiratory parameter of myocardium, brain and kidney was measured by oxygen electrode method. The protective effect of Reh on mitochondrion was observed in 2h po. Bepridil (Bep), an Ca 2+ channel blocker, ip 5 mg/kg 30 min prior to hypoxia served as positive control. Results In myocardium and kidney, hypoxia injury was showed similar, ST 3 and RCI value was increased as compared with the control group in a dose dependent manner, showing a selective stronger protection on renal mitochondrial than that on heart and brain. Conclusion Reh is effective in protecting metochondrial respiratory function in heart, brain and kidney from hypoxia and with the most potent effect on kidney.

关 键 词：线粒体 缺氧 苄普地尔 地黄 呼吸功能 动物模型 氧电极法 

分 类 号：R285.5[医药卫生—中药学]