RAPD法鉴定射干类中药  

Identification of Belamcanda chinensis and related Chinese materia medica of Iris L.by RAPD analysis

作　　者：黄芸[1] 秦民坚[1] 杨光[2] 徐珞珊[1] 周开亚[2] 

机构地区：[1]中国药科大学中药学院,江苏南京210038 [2]南京师范大学江苏省资源生物技术重点实验室,江苏南京210097

出　　处：《中草药》2002年第10期935-937,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金(3 0 170 10 3 );江苏省资源生物技术重点实验室资助项目;江苏省自然科学基金资助项目(BK970 85 )

摘　　要：目的　用 RAPD技术对射干类药材射干 Belamcanda chinensis及混淆品鸢尾属鸢尾 Iris tectorum、野鸢尾I.dichotoma、蝴蝶花 I.japonica、德国鸢尾 I.germanica等 5种药用植物进行分子水平的鉴定。方法　 CTAB法和 DNeasy TMPlant Mini Kit法提取射干类药材 5种原植物总 DNA,以随机引物进行随机扩增。结果　用 OPD- 0 8(5’- gtgtgcccca- 3')等作随机扩增引物进行随机扩增时 ,可得到识别这些物种基因组 DNA的多态片段。结论　RAPD法能有效的鉴别射干类药材 ,把射干和鸢尾、野鸢尾、蝴蝶花。Object To indentify the crude drugs of 'Shegan' Belamcanda chinensis (L.), DC.] and related Chinese materia medica of and related Chinese materia medica of Iris L., including Iris tectorum Maxim., I. dichotoma Pall., I. germanica L. and I. japonica Thunb. by RAPD technique. Methods General DNA was isolated from fresh leaves of B. chinensis and five plants of Iris L. by CTAB and DNeasy TM Plant Mini Kit. The random amplified polymorphic DNA (RAPD) was used to identify them. Results The polymorphic feather of DNA fingerprints of different species were compared by primer OPD 08. The studies showed that they have different relationships in heredity and the method of RAPD can be used to identify them easily. Conclusion Chinese materia medica of Iris L. can be idenlified by RAPD, and B. chinensis could be differentiated from I. tectorum, I. dichotoma, I. japonica, I. germanica.

关 键 词：射干 鸢尾属 RAPD DNA指纹图谱 药材鉴定 

分 类 号：R282.71[医药卫生—中药学] R282.5[医药卫生—中医学]