猪 2号染色体遗传连锁图谱的构建与QTL定位分析  

The Construction of the Genetic Map and QTL Locating Analysis on Chromosome 2 in Swine

作　　者：屈彦纯[1] 邓昌彦[1] 熊远著[1] 郑嵘[1] 余雳[1] 苏玉虹[1] 刘桂兰[1] 

机构地区：[1]华中农业大学畜牧兽医学院农业部猪遗传改良重点实验室,武汉430070

出　　处：《Acta Genetica Sinica》2002年第11期972-976,共5页

基　　金："973"项目资助 (编号" :G2 0 0 0 0 1610 5 );国家高技术研究发展计划专项经费资助 ~~

摘　　要：构建了猪 2号染色体的遗传连锁图谱 ,并进一步进行了重要生产性状数量性状位点的定位。结果表明 ,7个微卫星位点均为中高度多态性位点 ,多态信息含量为 0 .4 0 182～ 0 .5 84 77,可以满足遗传连锁图谱构建的要求。构建的资源家系遗传连锁图谱总长 15 2 .9cM ,位点的排列顺序与USDA结果一致 ,但除了Sw2 5 16与Sw12 0 1标记区间外 ,所有标记区间距离均大于USDA图谱。将连锁图谱与性状记录结合起来 ,进一步进行了猪数量性状位点定位的研究。在 2号染色体发现了显著影响活体估测瘦肉率等活体估测性状的QTLs ,此外还发现眼肌高度和背最长肌大理石纹的QTLs,其中影响活体估测瘦肉率的QTL达到了染色体显著的水平 (P <0 .0 1) ,且解释性状的表型变异达2 1.5 5 % ,影响眼肌高度和背最长肌大理石纹的QTLs分别可以解释 10 .12 %和 10 .97%的表型变异。影响活体估测性状的QTLs加性效应与显性效应作用方向相反 ,影响眼肌高度的QTL加性效应与显性效应相同 ,在大白猪中具有增效等位基因。定位的QTLs效应较大 ,为在群体中开展分子标记辅助育种奠定了理论基础。The study constructed the genetic linkage map of porcine chromosome 2 and further analysis of quantitative trait loci was conducted. The results of the study demonstrated that all 7 microsatellite loci we chose were with relatively high polymorphism, and its polymorphic information content was from 0.40182 to 0.58477. The genetic map we constructed for resource family was 152.9cM in length, with the order of all loci highly consistented with the USDA map. All marker intervals were longer than USDA map with the interval between marker Sw2516 and Sw1201 as an exception. Furthermore, we conducted QTLs locating analysis by combining the genetic map with the phenotypic data. QTLs affecting lively estimated traits such as lean meat percentage, were located at 60～65cM on chromosome 2, while QTLs for the height and marbling of Longissmus dorsi muscle were located at 20cM and 55cM, respectively Among them, QTL for estimated lean meat percentage was significant at chromosome wise level ( P<0.01 ) and was responsible for 21.55% of the phenotypic variance. QTLs for the height and marbling of Longissmus dorsi muscle were responsible for 10.12% and 10.97% of the phenotypic variance, respectively. The additive and dominance effect of lively estimated traits were in the inverse tendency ,while the QTL for the height of Longissmus dorsi muscle had its additive and dominance effect in the same tendency and was with advantageous allele in Large White. The QTLs wedetected had relatively large effect on phenotype and built a basis for molecular marker assisted selection and breeding.

关 键 词：猪 2号染色体 遗传连锁图谱 构建 QTL定位分析 

分 类 号：Q953[生物学—动物学]