与水稻籼粳分化有关的穗颈维管束性状基因的分子标记定位  

Molecular Mapping of Quantitative Trait Loci (QTL) for Characters of Vascular Bundles in Peduncle Related to indica-japonica Differentiation in Rice ( Oryza sativa L.)

作　　者：章志宏[1] 李平[2] 王玲霞[2] 胡中立[1] 朱英国[1] 朱立煌[3] 

机构地区：[1]武汉大学生命科学学院植物发育生物学国家教育部重点实验室,武汉430072 [2]四川农业大学水稻研究所,温江611130 [3]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《Acta Genetica Sinica》2002年第11期995-1000,共6页

基　　金：国家自然科学基金 (3 0 0 70 42 5 );教育部高等学校骨干教师资助计划项目 (2 0 0 0 65 ) ~~

摘　　要：水稻穗颈维管束数及其与穗一次枝梗数之比 (V/R)是与籼粳分化有关的重要性状。采用籼粳交 (圭 6 30 /0 2 4 2 8)杂种F1花药培养获得的DH群体 ,对水稻穗颈维管束数、穗一次枝梗数及V/R比进行了QTL分析。检测到 3个控制穗颈维管束数的QTL ;其中 ,效应最大的qVB 8的贡献率为 31.1% ,加性效应值为 1.96 ,增效等位基因来自籼稻亲本圭 6 30。 2个控制一次枝梗数的QTL效应较小 ,但分别与控制穗颈维管束数的 2个QTL同位。检测到影响V/R比的 3个QTL ;其中 ,效应最大的qV/R 1的贡献率为 2 5 .3% ,被定位于第 1染色体上 ,与落粒性基因sh 2紧密连锁 (亦或为一因多效 )。此外 ,还检测到 4对和 2对分别控制穗颈维管束数和V/R比的互作QTL。结果分析表明 ,水稻穗颈维管束数与穗一次枝梗数受不同的多基因系统控制 ,但这 2个多基因系统的某些位点在基因组中具有同位性 ;在第 1染色体上 ,控制V/R比、且效应最大的qV/R 1所在的染色体区段在水稻籼粳分化过程中可能具有重要作用。A doubled haploid population, consisting of 81 DH lines derived from the F 1 hybrid of a cross between an indica cultivar and a japonica cultivar, was used to map quantitative trait loci (QTL) controlling numbers of vascular bundles in peduncle, primary rachis branches and the ratio of vascular bundles to primary rachis branches (V/R ratio). For vascular bundles, three QTL were detected. Among them, the QTL qVB 8 with the largest effect individually accounted for 31.1% of the total variation. Two QTL controlling primary rachis branches were identified and they were co located with 2 of the 3 QTL for vascular bundles respectively. Three QTL for the V/R ratio were mapped on chromosome 1,2 and 8, respectively. Of the three QTL, the QTL qV/R 1 with the largest additive effect, explained 25.3% of the total variation, was located on chromosome 1 and found to be closely linked to the gene sh 2 , a major gene underlying grain shattering ability. In addition, four and two pairs of significant epistatic QTL were detected for vascular bundles and the V/R ratio, respectively, but none for rachis branches. Our results suggested that the numbers of vascular bundles and primary rachis branches were independently controlled by different polygenic systems, but the two polygenic systems shared a fraction of quantitative trait loci. It was also shown that the chromosome region carrying the QTL qV/R 1 on chromosome 1 might play an important role in the processes of indica japonica differentiation in rice.

关 键 词：水稻 籼粳分化 穗颈维管束 分子标记定位 RFLP标记 数量性状座位 

分 类 号：S511.2[农业科学—作物学]