一种新型淀粉酶的鉴定及其产酶菌株的筛选  

IDENTIFICATION OF A NEW TYPE OF AMYLASE AND MUTAGENESIS OF STRAIN ZX99 SECRETING THE ENZYME FOR PRODUCTION OF ISOMALTOOLIGOSACCHARIDE

作　　者：张应玖[1] 朱学军[1] 关键[2] 李吉平[2] 薛雁[1] 郝黎明[2] 赵文斌[2] 

机构地区：[1]吉林大学生命科学学院,长春130023 [2]解放军军需大学,长春130062

出　　处：《微生物学通报》2002年第5期38-41,共4页Microbiology China

基　　金：吉林省自然科学基金资助项目 (No 990 5 45 )

摘　　要：对筛选到的菌株ZX99产生的一种新型淀粉酶 (异麦芽低聚糖酶 )进行了分析鉴定。ZX99菌株能产生一种胞外淀粉酶 ,该酶能催化淀粉的降解产生异麦芽低聚糖。对原产酶菌株ZX99多次进行紫外线照射诱变后 ,获得了优良、稳定的变异菌株BS3 2 32 ,其产酶水平为原株的 1 60 %。产物薄层层析证明 ,该酶能催化淀粉的降解 ,产生异麦芽糖、潘糖、异麦芽三糖和异麦芽四糖等低聚糖 ,但对普鲁兰基本不起作用 ,由此证明它是一种不同于新型普鲁兰酶 (neopullulanase)和传统淀粉酶 (amylase)的一种新型淀粉酶。This paper reported a new type of amylase (neoamylase) secreted by a Bacillus strain ZX99. The enzyme was a kind of ectoenzyme that could catalyze starch into isomalto-oligosaccharide effectively, but could not act on pullulan as substrate. The strain Bacillus ZX99 was mutated by ultraviolet ray and a mutant strain BS3.232 was screened. The activity of the neoamylase produced from BS3.232 increased by 60% over that from ZX99 under the same conditions. The results of thin-layer chromatography of products from starch and pullulan catalyzed by the enzyme demonstrated that the enzyme was different from neopullulanase and can be used to produce isomaltooligosaccharide from starch, including isomaltose, panose, isomaltotriose, isomaltotetose.

关 键 词：新型淀粉酶 鉴定 产酶菌株 筛选 芽孢杆菌 

分 类 号：Q939.9[生物学—微生物学]