复合诱变筛选产细胞壁水解酶木霉菌株及产酶条件的优化  

STUDY ON SCREENING FOR TRICHODERMA VIRIDE BY COMPLICATION MUTAGENICITY:PRODUCTION OF CYTOHYDROLIST AND CULTURE CONDITION

作　　者：李蕤[1] 骆军[2] 虞磊[2] 鲁润龙[1] 潘仁瑞[1] 

机构地区：[1]中国科学技术大学生命科学学院,合肥230026 [2]合肥联合大学化学与生物工程系,合肥230022

出　　处：《微生物学通报》2002年第5期47-52,共6页Microbiology China

基　　金：安徽省自然科学基金资助项目 (No.982 41 5 1 5 )

摘　　要：利用复合诱变技术 ,筛选到一株产几丁质酶和葡聚糖酶能力较强的绿色木霉菌株(Trichodermaviride)LD 1 8。并对其产酶条件进行了优化 ,发现在麸皮∶诱导物∶麦秸粉 =2∶2∶7的固体培养基上 ,以 4g/L (NH4) 2 SO4为氮源 ,起始pH8 0 ,经 2 5℃培养 72h ,产生的真菌细胞壁水解酶 ,用以溶解食用菌、黑曲霉等丝状菌丝体的细胞壁 ,制备原生质体 ,效果较优。A strain LD-18 which produced chitinase and glucanase was screened by mutagenicity method and identified as Trichoderma viride . Effects of medium composition on producing cytohydrolist were investigated. LD-18 was cultured on complex medium in which wheat bran:inducer:straw powder is 2∶2∶7 as carbon source and 4g/L(NH) 2SO 4 as nitrogen source, and the culture conditions are initial pH8.0, 25℃ and 72h. The protoplast formations of domestic fungus and Aspergillus niger prepared by the cytohydrolist is the more efficient.

关 键 词：复合诱变筛选 变细胞壁水解酶 木霉菌株 产酶条件 优化 

分 类 号：Q939.9[生物学—微生物学]