热带假丝酵母酰基辅酶A氧化酶的活性测定方法  

ACTIVITY ASSAY OF ACYL COENZYME A OXIDASE(ACO) FROM CANDIDA TROPICALIS

作　　者：欧阳晶[1] 陈远童[1] 

机构地区：[1]中国科学院微生物学研究所,北京100080

出　　处：《微生物学通报》2002年第5期61-65,共5页Microbiology China

基　　金："九五"国家攻关基金资助项目 (No 96 C0 3 0 3 0 4)

摘　　要：酰基辅酶A氧化酶 (ACO)是热带假丝酵母内二元酸β氧化的限速酶。工业上利用烷烃生产二元酸时 ,使用ACO低活性的菌株 ,将有利于提高二元酸的产量和纯度。本文以热带假丝酵母为材料 ,在Allain方法基础上加以改进 ,可简便、准确地测定ACO酶活性 ,为二元酸生产菌株的筛选方法提供了量化标准 ,并为进一步研究此酶的酶学性质奠定了基础。对本实验室所保存的一系列具有不同产酸能力的热带假丝酵母菌种进行酶活性测定 ,发现酶活性与产酸能力有明显的相关性。Acyl Coenzyme A Oxidase(ACO) is the rate-limiting enzyme of the β-oxidation of dicarboxylic acid in Candida tropicalis . Using strains with low Acyl Coenzyme A Oxidase activity to produce dicarboxylic acid on an industrial scale will facilitates the improvement of the yield and purity of dicarboxylic acid. In this research, we improved the method of Allain, so as to determine the activity of Acyl Coenzyme A Oxidase from Candida tropicalis easily and correctly. This method can be used as quantity criterion in the screen of industrial strains of dicarboxylic acid production, and this work established the basic of character study of Acyl Coenzyme A Oxidase. We determined the activities of Acyl Coenzyme A Oxidase from a series of Candida tropicalis strains with different ability of dicarboxylic acid production, and found the obvious relationship of ACO activity and ability of acid production.

关 键 词：热带假丝酵 酰基辅酶A氧化酶 测定方法 酶活性 二元酸 

分 类 号：Q936[生物学—微生物学]