兔精子发生过程中cyclin B1基因表达和蛋白定位的发育阶段依赖性  

Developmental stage-dependence of cyclin B1 protein localization and gene expression in rabbit spermatogenesis

作　　者：孔维华[1] 颜山[2] 顾正[2] 左嘉客[1] 

机构地区：[1]山东大学生命科学学院,济南250100 [2]上海市计划生育科学研究所计划生育药具国家重点实验室,上海200032

出　　处：《生理学报》2002年第5期400-404,共5页Acta Physiologica Sinica

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 3 9770 3 70 ;3 963 0 160 )

摘　　要：利用原位杂交和免疫组化等方法 ,研究兔精子发生过程中生精细胞cyclinB1mRNA的表达和蛋白定位特点。结果显示 :兔生精上皮中cyclinB1mRNA主要分布在初级精母细胞中 ,直至圆形精子细胞仍然存在 ,于精子细胞的变态过程中逐渐消失。在伸长的精子细胞和精子中未检测出cyclinB1mRNA。CyclinB1蛋白在进入分裂期的精原细胞和精母细胞中表达 ;在圆形精子细胞和伸长的精子细胞中呈现大量的cyclinB1蛋白。上述结果表明 :在兔精子发生过程中 。By means of in situ hybridization and immunohistochemistry, the protein localization and gene expression of cyclin B1 in spermatogenic cells were characterized during the spermatogenesis of rabbits The results showed that the cyclin B1 mRNA in rabbit spermatogenic epithelium was expressed dominantly in primary spermatocytes The expression was observed in round spermatids with a gradual decline in the process of metamorphosis of the spermatids, but not in elongated spermatids and sperms Cyclin B1 protein was expressed in mitotic spermatogonia and meiotic spermatocytes and was observed predominantly in round and elongated spermatids These results indicate that the expression of cyclin B1 mRNA and localization of cyclin B1 protein are dependent on the developmental stages of spermatogenic cells

关 键 词：兔 精子发生过程 CYCLINB1 基因表达 蛋白定位 生精细胞 发育阶段依赖性 

分 类 号：Q492[生物学—生理学]