阿魏酸钠对氧化损伤大鼠晶状体上皮细胞凋亡的防护  

Sodium ferulate protecting against apoptosis of lens epithelial cell injured by oxidation

作　　者：祁明信[1] 黄秀榕[2] 汪朝阳[2] 王勇[1] 

机构地区：[1]福建中医学院附属第二人民医院,福建福州350003 [2]福建中医学院病理生理研究中心,福建福州350003

出　　处：《中国药理学与毒理学杂志》2002年第5期358-362,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家中医药管理局科研基金资助重点课题(97Z0 34);福建省教育厅科学基金资助项目 (K2 0 0 10 91)

摘　　要：目的　探讨阿魏酸钠 (SF)对实验性氧化损伤大鼠晶状体上皮细胞 (LEC)凋亡有无抑制作用。方法　采用无菌操作摘取SD大鼠双眼 ,并在手术显微镜下分离晶状体 ,随机分成空白对照组、过氧化氢组 (H2 O2 )、吡诺克辛 (PS)组和SF组。使晶状体孵育在 30 0 μmol·L- 1H2 O2 培养液中复制LEC凋亡模型 ,同时加入终浓度为 5mmol·L- 1的SF ,置CO2 培养箱共同孵育 2 4h。取晶状体前囊膜 ,采用TUNEL法检测LEC凋亡及凋亡率、透射电子显微镜观察LEC超微结构改变和凋亡小体形成。结果　H2 O2组LEC凋亡率 (92 .0± 2 .6 ) %显著高于空白对照组(3.5± 1.8) %;SF组LEC凋亡率 (2 0 .8± 3.0 ) %显著低于H2 O2 组 ,且与PS组 (5 6 .0± 9.9) %有非常显著的差异。超微结构研究显示 ,H2 O2 组绝大多数LEC发生凋亡 ,并呈凋亡各期严重改变的全过程 ;SF组仅少数LEC发生凋亡 ,并多为早期或中期的轻微改变。结论　SF可明显抑制实验性氧化损伤大鼠LEC凋亡 ,且明显优于PS滴眼液。AIM To investigate if sodium ferulate inhibits the apoptosis of lens epithelial cell (LEC) resulted from the experimental oxidative injury. METHODS Eyes in SD rats were excised and lenses were separated under operating microscope and sterilized condition. Lenses were divided randomly into four groups: control group, hydrogen peroxide group (H 2O 2), pirenoxine sodium group (PS) and sodium ferulate group (SF). Lenses were incubated in CO 2 incubator for 24 h with 300 μmol·L -1 H 2O 2 and with or without 5.3 mg·L -1 PS or 5 mmol·L -1 SF. LEC apoptosis and apoptosis rate were measured by terminal deoxynucleotidyl transferase mediated biotin dUTP nick end labeling (TUNEL) method. Ultrastructure changes and apoptosis bodies of LEC were observed under transmission electron microscope. RESULTS The results showed: ① Apoptosis rate in H 2O 2 group (92.0±2.6)% was significantly higher than that in control group (3.5±1.8)%. Apoptosis rate in SF group (20.8±3.0)% was remarkably lower than that in H 2O 2 group and PS group. ② Ultrastructure observation indicated that apoptosis cells occurred in most LEC of H 2O 2 group and the changes were severe and presenting different stages. While a few apoptosis cells were observed in SF group, the changes were slight and most of them were in early and middle stages. CONCLUSION The study indicated that SF inhibited apoptosis of LEC of experimental oxidative injury significantly, the effects were stronger than PS.

关 键 词：晶状体 上皮细胞 抗氧化药 阿魏酸钠 白内障 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]