亚砷酸钠和氧苯胂对人皮肤成纤维细胞缝隙连接通讯的动态影响及作用特征  

Effect of arsenite and phenylarsine oxide on gap junctional intercellular communication between human skin fibroblasts

作　　者：邓芙蓉[1] 李艳宏[1] 张华明[1] 郭新彪[1] 

机构地区：[1]北京大学公共卫生学院劳动卫生与环境卫生学系,北京100083

出　　处：《中国药理学与毒理学杂志》2002年第5期378-381,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：美国中华医学基金专项人才基金资助项目(ORF980 4 )

摘　　要：目的　了解和比较不同砷化合物对细胞缝隙连接通讯的动态影响及作用特征 ,进一步阐明砷的致癌机理。方法　用激光共聚焦显微镜 ,以细胞漂白后荧光强度的恢复作为评价缝隙连接通讯的指标 ,按激光漂白后荧光恢复实验技术测定亚砷酸钠和氧苯胂对人皮肤成纤维细胞缝隙连接通讯的影响。结果　亚砷酸钠和氧苯胂均可明显抑制激光漂白后细胞荧光强度的恢复 ,其作用有明显的剂量 反应关系 ;进一步的研究发现 ,在去除亚砷酸钠作用后的不同时间 ,实验组细胞荧光强度恢复增加值显著低于对照组 ,表明细胞缝隙连接通讯功能没有恢复 ;而在去除氧苯胂作用 4h后 ,实验组荧光强度恢复增加值与对照组无显著性差异 ,表明细胞缝隙连接通讯有所恢复。结论　无机砷和有机砷均可明显抑制细胞缝隙连接通讯 ,但二者的作用特征有所不同。AIM To investigate and compare the dynamic effect of arsenic compounds on gap junctional intercellular communication(GJIC) between human skin fibroblasts, and elucidate the mechanism of arsenic carcinogenesis. METHODS GJIC between skin fibroblasts was measured by fluorescence redistribution after photobleaching (FRAP). RESULTS Arsenite at concentrations from 0.05-5.0 μmol·L -1 and PhAsO at concentrations from 0.1-10 nmol·L -1 significantly inhibited the fluorescence redistribution between skin fibroblasts in a dose dependent manner(P<0.01). Further study indicated that GJIC between skin fibroblasts recovered after PhAsO was removed, but the GJIC didn′t recover when arsenite was removed. CONCLUSION Our results suggested that arsenite and PhAsO significantly inhibit GJIC between human skin fibroblasts, but their action mode is different.

关 键 词：皮肤 成纤维细胞 亚砷酸钠 氧苯胂 细胞缝隙连接通讯 砷化合物 致癌机理 

分 类 号：R114[医药卫生—卫生毒理学]