胸腺来源免疫抑制提取物对不同组织肿瘤细胞作用的研究  

Effect of thymus derived immunosuppressive extract on the proliferation of different type tumor cells in vitro

作　　者：吴国庆[1] 王凤山[2] 吴晓燕[1] 苗乃法[1] 

机构地区：[1]潍坊医学院免疫学研究所,山东潍坊261042 [2]山东大学药学院生化与生物技术药物研究所,山东济南250012

出　　处：《中国生化药物杂志》2002年第5期234-235,共2页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的探讨小牛胸腺来源免疫抑制提取物 (TISE c)对不同组织来源肿瘤细胞的影响。方法采用3 H TdR掺入方法 ,检测TISE c对肝癌、肺腺癌、膀胱癌传代细胞株细胞增生的影响 ,以及在显微镜下观测TISE c对癌细胞形态学变化的影响。结果TISE c对 3种肿瘤细胞均具有抑制分裂增生作用 ,但是癌细胞形态正常。结论TISE c可能是一种能够抑制癌细胞分裂增生的生物制剂 ;对不同组织来源的癌细胞不存在组织特异性 ;PurposeTo explore the effects of calf thymus derived immunosuppressive extract(TISE c) on the proliferation of different tumor lineages.MethodsThree lineages of cancer cells, hepatocarcinoma(HEPG2), andenocarcinoma of lung(GLC) and carcinoma of urinary bladdder(E J), were incubated with TISE c; the cell proliferation was detected with H 3 thymidine uptake and the cell morphology was examined under microscope.ResultsTISE c could inhibit the proliferation of the three cancer lineages, but their morphology was normal.ConclusionTISE could have an inhibitory effect on the proliferation of cancer cells without tissue specificity through non cytotoxicity mechanism.

关 键 词：胸腺免疫抑制提取物 肿瘤细胞 细胞增生 抑制作用 

分 类 号：R392－33[医药卫生—免疫学] R730.5[医药卫生—基础医学]