头穴透刺对急性脑梗塞大鼠脑组织中β-EP与cAMP含量的相关性研究  

Effects of Scalp-point Penetration Needling on Cerebral β-EP and cAMP Contents and the Correlation between β-EP and cAMP in Rats with Acute Cerebral Infarction

作　　者：吴绪平[1] 韩肖华[1] 王亚文[1] 刘又香[1] 

机构地区：[1]湖北中医学院,武汉430061

出　　处：《针刺研究》2002年第3期170-173,共4页Acupuncture Research

基　　金：湖北省科委重点科技项目 (NO .971P140 1)

摘　　要：目的 :探讨头穴透刺对急性脑梗塞大鼠脑组织中β EP( β Endorphin ,β内啡肽 )与cAMP(cyclicAMP ,环磷腺苷 )含量变化的相关性。方法 :用线栓法直接阻断大鼠大脑中动脉制作急性脑梗塞模型 ,采用放免技术 ,检测头穴透刺对急性脑梗塞大鼠脑组织中 β EP与cAMP含量的影响。结果 :头穴透刺后可使大鼠脑组织中cAMP的含量明显升高 ,使β EP含量明显降低而趋于正常值 ,两者之间呈显著负相关。结论 :头穴透刺可以调节脑组织中 β EP与cAMP的含量 ,改善梗死脑组织供血 。Objective: To observe the effect of scalp-acupuncture on cerebral β-EP and cAMP contents and to analyze the correlation between changes of β-EP and cAMP in rats with acute cerebral infarction. Methods: 90 SD rats were randomly divided into control group (n=10), model group (n=40) and scalp-acupuncture group (n=40), those of the later two groups were respectively divided into 4 subgroups further, with 10 cases in every subgroup. Penetration needling from 'Baihui' (GV 20) to 'Qianding' (GV 21) and from 'Shuaigu' (GB 8) to 'Xuanli' (GB 6) was performed using filiform needles and the scalp-acupoints were stimulated with G6805 Electroacupuncture Therapeutic Apparatus (1 mA, 30 Hz and duration of 30 min), once after waking up, twice daily afterwards. Acute cerebral infarction model was produced by blocking the blood flow of the middle cerebral artery with a cauterized global nylon thread end (0.25 mm in diameter). The contents of β-EP and cAMP in the brain tissues of rats were assayed with radioimmunoassay (RIA). Results: In model group (including 6 hr, 24 hr, 48 hr and 72 hr subgroups), cAMP contents were markedly lower than that of normal control group; while β-EP contents were significantly higher than that of normal control group (P<0.01). After acupuncture, the content of cAMP increased significantly and that of β-EP lowered evidently in comparison with model group (P<0.05, 0.01), which were close to those of normal control group. There was a significant negative correlation between the contents of β-EP and cAMP (r=-0.8583). Conclusion: Electroacupuncture of scalp-acupoint can adjust the contents of β-EP and cAMP in the brain tissues of rats with acute cerebral infarction, which may contribute to the effect of acupuncture in improving symptoms and signs of cerebral infarction patients.

关 键 词：脑梗塞 头针透刺 电针 Β-EP CAMP 

分 类 号：R277.733.3[医药卫生—中医学] R246.6