间接荧光抗体法快速诊断海兰褐蛋鸡J亚群禽白血病的研究  

Rapid diagnosis of avian leukosis subgroup J of egg-type chicken by indirect fluorescent assay

作　　者：徐镔蕊[1] 董卫星[2] 何召庆[1] 冯小宇[1] 余春明[1] 张丽[1] 任艳丽[1] L F LEE Mao-xiang LI 

机构地区：[1]中国农业大学动物医学院,北京海淀100094 [2]安徽省农业委员会畜牧局,安徽合肥230001 [3]美国农业部禽病与肿瘤研究所

出　　处：《中国兽医杂志》2002年第9期7-9,共3页Chinese Journal of Veterinary Medicine

摘　　要：某地区海兰褐蛋鸡发生肿瘤性传染病 ,经临床症状和病理学检查 ,在肝、脾、肾、卵巢、输卵管、肺、骨髓、腺胃、肠道等可见胞浆内充满球形嗜酸性颗粒的骨髓瘤细胞 ,呈灶状或弥漫性的分布。该变化是禽骨髓细胞瘤病病理学的特征性变化 ,具有证病意义。经用特异性抗 J亚群白血病病毒囊膜蛋白 gp85的单克隆抗体的免疫组化方法 ,在病料组织切片上检出病毒抗原 ,从而确诊为蛋鸡 J亚群禽白血病。本研究是尝试用间接荧光抗体法来快速诊断该病。采用特异性抗 J亚群禽白血病病毒囊膜蛋白 gp85单克隆抗体对组织触片作间接荧光抗体试验 ,检测 J亚群禽白血病病毒抗原。在骨髓、食管、肌肉、输卵管、肾都出现了范围广而且很明亮的荧光 ;肝、肺、脾的荧光较为明亮 ;心脏的荧光较弱 ,但清晰可见。表明在病料组织中存在特异性病毒抗原。A neoplastic disease took place in a egg type chicken named Hyline brown.By clinical symptom and pathologic examine,it was found that there were multifocal or widespread marrow tumor cells filled with ball like acidophilic particles in cytosol from the liver, spleen, kidney, ovarium, oviduct, lung, marrow, ventriculus glandularis and gut.These are the characteristic pathology changes of avian leukosis, and have the values of diagnosis. With immunohistochemistry of special monoclonal antibody against gp85 Envelope Protein of Avian Leukosis Virus Subgroup J,antigen of virus was found in section,thus it was diagnosed to be ALV J. The essay is experimented to rapidly diagnose ALV J with IFA.Indirect Fluorescent Assay (IFA) was carried out to examine ALV J in the microscope slide of specimen with McAb against gp85 from ALV J. The marrow, oesophagus, muscle, fallopian tube, kidney all appeared the wide and very bright fluorescence; The fluorescence of the liver, lung, spleen are bright too; The heart's fluorescence is weak, but clear. Therefore it revealed that special antigen of virus is in the specimen.

关 键 词：诊断 蛋鸡 J亚群禽白血病 间接荧光试验 单克隆抗体 抗体检测 

分 类 号：S858.31[农业科学—临床兽医学]