天花粉蛋白基因的克隆、表达和活性鉴定  

Cloning, Expression and Characterization of Trichosanthin Gene

作　　者：蔡煊[1] 胡拥军[2] 徐洪[2] 俞吉安[1] 孙兵[2] 

机构地区：[1]上海交通大学生命科学技术学院,上海200030 [2]中国科学院上海生物化学和细胞生物学研究所,上海200030

出　　处：《上海交通大学学报》2002年第10期1503-1505,共3页Journal of Shanghai Jiaotong University

摘　　要：用 PCR( Polymerase Chain Reaction)扩增出天花粉蛋白 ( Trichosanthin,TCS)基因全长序列 ,插入表达载体 .经双酶切鉴定及测序分析 ,表明阅读框正确 .表达 His- TCS活性融合蛋白并纯化天花粉蛋白 ,鉴定出其具有 RNATCS (Trichosanthin) gene was cloned into an expression vector. The plasmid was extracted and digested with restriction enzymes. The result of the sequence analysis shows the TCS whole length gene is obtained. The TCS protein was expressed and purified. The TCS protein's biological activity of RNA N glycosidase was characterized. ELISA shows that this protein can bind with specific antibody TE1(IgE).

关 键 词：天花粉蛋白 基因克隆 分离纯化 生物活性 基因表达 RNA N-糖苷酶活性 抗原抗体结合活性 

分 类 号：Q78[生物学—分子生物学]