天麻对铅所致大鼠海马损害的拮抗作用  

The antagonism of rhizoma gastrodiae to lead-induced damage of hippocampus in rats

作　　者：李茂进 胡俊峰[2] 李国珍 谢林 

机构地区：[1]山东省劳动卫生研究所毒理室,济南250062 [2]中国疾病预防控制中心健康教育所 [3]北京大学医学部公共卫生学院劳动卫生与环境卫生教研室

出　　处：《中华劳动卫生职业病杂志》2002年第5期331-333,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　观察并探讨天麻对铅所致学习记忆损害的拮抗作用。方法　选用 36只Wistar大鼠 ,随机分为 3组 ,每组 12只。 (1)对照组 :给予蒸馏水 ;(2 )染铅组 :醋酸铅 (0 .1g·kg-1·d-1) ;(3)天麻铅组 :醋酸铅 (0 .1g·kg-1·d-1) +天麻 (4.0g·kg-1·d-1)。每月用游泳试验测试学习记忆 1次。 3个月后处死大鼠 ,分别测定大鼠海马一氧化氮 (NO)和总抗氧化能力 (TAOC)并进行病理检查。结果　 (1)在游泳试验中 ,染铅组大鼠 5min内到达平台次数 (1、2、3月分别为 :10 .1± 1.10、7.8± 1.30、5 .4±0 .97)低于对照组 ,差异有显著性 (P <0 .0 1) ;天麻铅组大鼠到达平台的次数 (1、2、3月分别为 :11.9±0 .95、10 .9± 0 .95、9.7± 0 .96 )高于染铅组 ,差异有显著性 (P <0 .0 1)。 (2 )染铅组大鼠海马NO含量为(0 .733± 0 .0 15 ) μmol/gpro和TAOC为 (0 .94 5± 0 .0 17)U/mgpro ,低于对照组 ,差异有显著性 (P <0 .0 1) ;天麻铅组海马NO含量为 (0 .76 9± 0 .0 2 1) μmol/gpro和TAOC为 (0 .986± 0 .0 10 )U/mgpro ,高于染铅组 ,差异有显著性 (P <0 .0 1)。 (3)病理检查 :染铅组大鼠海马明显萎缩 ,细胞变性坏死、脱失明显 ,轴突溶解或消失 ;天麻铅组海马萎缩不明显 ,细胞形态基本正常 ,无明显坏死、脱失现象 ,轴?Objective To observe and explore the antagonism of rhizoma gastrodiae(RGT) to the impairment of learning and memory ability induced by lead in rats. Methods 36 Wistar rats were randomly divided into 3 groups,12 rats in every group,(1)control group:treated with distilled water;(2)lead group:treated with lead acetate(0.1 g·kg -1·d -1);(3)lead-RGT group:lead acetate(0.1 g·kg -1·d -1)+RGT(4.0 g·kg -1·d -1).The ability of learning and memory of the rats was measured monthly by swimming test;3 months later,the rats were decapitated and nitric oxide(NO) and total antioxidative capacity(TAOC) in hippocampus were measured immediately and the examination for pathology was also made. Results (1)In swimming test,the number of seeking for anchorage in lead group(1,2,3 month:10.10±1.10,7.80±1.32,5.40±0.97 respectively) were significantly decreased,compared with the control(P<0.01);the number of seeking for anchorage in RGT-lead group(1,2,3 month:11.90±0.95,10.90±0.95,9.70±0.96 respectively) were significantly increased,compared with lead group(P<0.01).(2)NO(0.733±0.015)μmol/g pro and TAOC(0.945±0.017)U/mg pro in hippocampus of lead group were significantly decreased compared with the control(P<0.01) whereas NO(0.769±0.021)μmol/g pro and TAOC(0.986±0.010)U/mg pro in hippocampus of RGT-lead group were significantly increased compared with lead group(P<0.01).(3)Pathological examination showed that in lead group,marked atrophy in hippocampus,cellular denaturation and necrosis,dissolution and disappearance in axon were found.In lead-RGT group,the atrophy of hippocampus was not obvious,the cell morphology was nearly normal,no obvious abnormal changes were seen. Conclusion RGT is antagonistic to the impairment of hippocampus and of learning and memory ability induced by lead.

关 键 词：拮抗作用 铅 天麻 学习记忆 一氧化氮 总抗氧化能力 动物实验 

分 类 号：R114[医药卫生—卫生毒理学]