胰岛素样生长因子Ⅱ基因在大鼠INS-1细胞中表达的调控  

Regulation of insulin-like growth factor-Ⅱ gene expression in rat INS-1 cells

作　　者：德伟[1] 吴月[1] 徐桦[1] 程志祥[1] 熊俊[1] Paul Czernichow 

机构地区：[1]南京医科大学生物化学与分子生物学系,210029 [2]INSERM U457,H■pital Robert Debr

出　　处：《中华内分泌代谢杂志》2002年第5期384-387,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：江苏省自然科学基金资助项目 (BK2 0 0 1119)

摘　　要：目的　研究不同浓度葡萄糖对INS 1细胞IGF Ⅱ基因表达的调节及对INS 1细胞增殖的作用 ,并比较其对IGF Ⅱ与胰岛素基因表达的关系。方法　采用Northern杂交分析IGF ⅡmRNA表达 ,用3 H 胸腺嘧啶掺入法检测细胞增生。结果　IGF Ⅱ在INS 1细胞中表达。当葡萄糖浓度为 10～ 2 0mmol/L时 ,IGF ⅡmRNA表达量提高 3倍以上。佛波醇十四乙酸对INS 1细胞的IGF ⅡmRNA的表达及增生不起作用 ,而Forskolin可抑制INS 1细胞的IGF ⅡmRNA表达。结论　高浓度葡萄糖促进INS 1细胞中IGF ⅡmRNA表达 ,低浓度的葡萄糖环境中胰岛素mRNA的表达量高。Objective To study the effects of different glucose concentrations on IGF Ⅱ gene expression and cell proliferation in INS 1 cells and to compare the effects of glucose on IGF Ⅱ and insulin gene expressions. Methods The IGF Ⅱ and insulin gene expressions were determined by Northern blot. The cell proliferation was assessed by 3H thymidine incorporation assay. Results IGF Ⅱ gene mRNA was expressed in INS 1 cell line. IGF Ⅱ mRNA was increased more than three folds at glucose concentration of 10～20 mmol/L. Phorbol 12 myristate 13 acetate (PMA) had no effect on IGF Ⅱ gene expression and proliferation in INS 1 cells, and Forskolin suppressed the stimulatory effects of glucose on the IGF Ⅱ mRNA expression. Conclusion In higher glucose concentration IGF Ⅱ mRNA is highly expressed, while insulin mRNA is highly expressed in lower glucose concentration.

关 键 词：胰岛素样生长因子Ⅱ 胰岛素 葡萄糖 基因表达 

分 类 号：R346[医药卫生—基础医学]