耐低pH的氧化亚铁硫杆菌选育及其氧化硫酸亚铁的初步研究  

Adaption of Thiobacillus ferrooxidans Tolerating Low pH and Biooxidation of Ferrous Sulphate

作　　者：龙中儿[1,2] 黄运红[3] 蔡昭铃[1] 丛威[1] 欧阳藩[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室 [2]江西师范大学化学与生物科学学院,江西南昌330027 [3]江西师范大学化学与生物科学学院

出　　处：《过程工程学报》2002年第5期415-420,共6页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金重点资助项目(编号:59834150)

摘　　要：低pH条件下高效氧化硫酸亚铁的氧化亚铁硫杆菌的选育是两步法生物浸出工艺应用的前提条件. 本文经定向培育和连续培养筛选得到pH为1.7～2.0条件下氧化硫酸亚铁的氧化亚铁硫杆菌驯化菌,其氧化硫酸亚铁的最适pH值由原来的2.0～3.0下降到1.7～2.0,在初始pH为1.7条件下培养48 h后,9K培养基中Fe2+氧化率从13.1%提高到85%. 动力学研究结果表明,该菌在250 ml摇瓶中装量100 ml、接种量10%、起始pH=1.7、温度31oC、转速120 r/min的优化条件下培养52 h后,9K培养基中Fe2+氧化率在98%以上,对数期的细菌比生长速率为0.0635 h(1,与出发菌株相近. 该菌有望用于两步法生物浸出矿物的新工艺.Thiobacillus ferrooxidans oxidating ferrous sulphate effectively at low pH (pH<2.0) is prerequisite to the industrial application of two-step bioleaching. In this work, the strain was adapted by sequential adaptive culture and isolation via continuous culture. Compared with the original Thiobacillus ferrooxidans TF5, its optimum pH for oxidating ferrous iron was changed from 2.0~3.0 to 1.7~2.0, and the ratio of oxidized ferrous iron was improved from 13.1% to 85% at pH=1.7. Kinetic research showed that, under the optimized cultural condition of initial pH=1.7, culture temperature near 31oC, 98% of ferrous iron contained in 9K medium was oxidized after cultivation for 52 h in a 250 ml Erlenmeyer flask incubated on a rotary shaker at 120 r/min, and the growth rate of Thiobacillus ferrooxidans cells was 0.0635 h-1, which is similar to the original type. It was showed that the adapted Thiobacillus ferrooxidans is potentially useful in the industrial application of two-step bioleaching.

关 键 词：氧化亚铁硫杆菌 选育 低PH值 硫酸亚铁 生物氧化 

分 类 号：TF18[冶金工程—冶金物理化学]