盐藻在气升式光生物反应器中的光自养培养  

Cultivation of Dunaliella salina in an Airlift Photobioreactor

作　　者：郝建欣[1] 丛威[1] 康瑞娟[1] 蔡昭铃[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100080

出　　处：《过程工程学报》2002年第5期443-447,共5页The Chinese Journal of Process Engineering

摘　　要：在气升式光生物反应器中进行了盐藻培养特性的研究，确定了盐藻在2.5 L气升式光生物反应器中培养的适宜条件为：温度30oC，光强1.6 mW/cm2，盐浓度16%，通气量20 ml/min. 扩大到20 L反应器培养盐藻生长良好. 采用气升式光反应器培养盐藻生长快，周期短，4~7 d后即可进入稳定期；最终细胞密度大，最大为1.6′106 cells/ml；藻液中胡萝卜素含量高，最高含量32 mg/L.实验表明气升式光生物反应器适合于盐藻的培养.The growth of Dunaleilla salina in an airlift photobioreactor was examined. From the experiments the suitable growth conditions were obtained: temperature of 30oC, light intensity 1.6 mW/cm2, NaCl concentration 12%, CO2 concentration about 6% in mixed feed gas, and aerating rate 20 ml/min. The growth rate of D. salina in the airlift photobioreactor was higher than in the flask, the maximum specific growth rate was 4.16 d-1. The cultivation was completed within 4~7 d, the maximum cell density was 16105 cells/ml, and the highest carotene concentration up to 32 mg/L.

关 键 词：光自养培养 气升式光生物反应器 盐藻 胡萝卜素 

分 类 号：Q949.21[生物学—植物学]