抗肿瘤药物体外诱导新鲜癌组织及其外周血淋巴细胞mdr-1基因表达的相关性研究  

STUDY ON THE RELATIVITY OF mdr-1 EXPRESSION INDUCED BY DRUG BETWEEN FRESH TUMOUR CELLS AND THE PERIPHERAL LYMPHOCYTES IN VITRO

作　　者：何琳[1] 张巧[2] 胡杰英[1] 

机构地区：[1]河南省肿瘤研究所分子生物学研究室,河南郑州450003 [2]郑州大学公共卫生学院,河南郑州450052

出　　处：《癌变．畸变．突变》2002年第4期229-231,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河南省科技攻关项目资助课题 (9712 0 0 3 0 0 0 4)

摘　　要：目的 :通过对恶性肿瘤患者的癌组织细胞及其外周血淋巴细胞进行抗肿瘤药物的体外诱导 ,用RT PCR法检测其mdr 1基因表达状况 ,从而找出两者之间的相关性 ,从基因水平解决非手术癌患者化疗用药个体化。方法 :在采集手术标本的同时抽取该患者外周血 ,用 8种抗肿瘤药物进行体外诱导 ,RT PCR法检测 32例恶性肿瘤患者癌组织细胞及其外周血淋巴细胞的mdr 1基因表达状况。利用SAS软件计算两者之间的相关关系。结果 :32例恶性肿瘤患者的新鲜癌组织细胞与其外周血淋巴细胞的抗肿瘤药物体外诱导mdr 1基因表达呈正相关关系。结论 :对于失去手术机会或病灶很小不能取材的患者可以用外周血淋巴细胞替代癌细胞做RT PCR体外药敏检测。Purpose: To investigate the relativity of mdr 1 expression between the fresh tumour cells and the peripheral lymphocytes of 32 patients with malignant tumours. Methods: Using reverse transcription polymerase chain reaction (RT PCR) assay, mdr 1 expression induced by 8 anti cancer drugs in the fresh tumour cells and the peripheral lymphocytes of 32 patients with malignant tumours was detected. Results: There was a positive relativity of mdr 1 expression between the fresh tumour cells and the lymphocytes. Conclution: The lymphocytes of peripheral blood can replace tumour cells in selecting sensitive drugs with RT PCR assay in vitro. It can provide a new way of using drug individually for patients with malignant tumours.

关 键 词：抗肿瘤药物 体外诱导 癌组织 外周血淋巴细胞 MDR-1基因 相关性 RT-PCR 

分 类 号：R979.1[医药卫生—药品] R730.53[医药卫生—药学]