胆汁胆固醇对胆囊收缩素受体表达的影响  

Effect of cholesterol in bile on cholecystokinin receptor in the gallbladder

作　　者：傅华群[1] 吴伟顶[1] 邹书兵[1] 黄明文[1] 黄长文[1] 徐艳萍[1] 

机构地区：[1]江西医学院第二附属医院肝胆外科,南昌330006

出　　处：《中华外科杂志》2002年第10期786-788,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目 (3 9960 0 84)

摘　　要：目的　探讨胆汁胆固醇对胆囊收缩素受体 (CCK R)表达的影响。　方法　采用放射免疫分析法和受体放射配基结合法检测对照组 (n =2 5 )、高胆固醇组 (n =2 5 )、自然恢复组 (n =2 5 )及治疗组 (n =2 5 )豚鼠门静脉血CCK水平、胆囊CCK R的最大结合容量 (Bmax)和亲和力 (Kd) ,同时观察空腹胆囊体积 (FV)、胆囊胆汁量 (FB)和餐后胆囊体积 (RV)、胆囊胆汁量 (RB)及胆囊收缩率 (E)、胆汁胆固醇浓度的变化。　结果　与对照组比较 ,高胆固醇组豚鼠FV[(0 89± 0 2 6 )～ (1 34± 0 6 1)cm3 ]、FB[(0 6 8± 0 2 0 )～ (1 0 1± 0 4 3)cm3 ]、RV[(0 2 8± 0 0 8)～ (0 90± 0 5 3)cm3 ]、RB[(0 2 3± 0 0 6 )～(0 83± 0 32 )cm3 ]增大 ,E[(6 5 83± 7 32 ) %～ (47 2 2± 5 2 4 ) % ]下降 ,胆汁胆固醇浓度 [(0 4 4±0 11)～ (0 6 0± 0 13)mmol/L]升高 ,门静脉血CCK水平及CCK R的Kd无改变 ,而CCK R的Bmax[(6 0± 2 7)～ (32± 13)fmol/mg蛋白 ]下降 ;与自然恢复组比较 ,治疗组上述各项指标正常。　结论　胆汁中的高胆固醇通过下调胆囊CCK R表达而导致胆囊收缩功能障碍 ,降低胆汁高胆固醇浓度可以促进胆囊动力功能的恢复。Objective To assess the effect of cholesterol in bile on cholecystokinin receptor(CCK R) in the gallbladder. Methods One hundred Guinea pigs were randomly divided into four groups, 25 animals for each. The control group was fed a standard diet, and the cholesterol group fed a diet containing 2% cholesterol. After taking the 2% cholesterol diet for two weeks, the natural group persisted on the standard diet, and the treated group was perfused by traditional Chinese medicine Serum cholecystokinin(CCK) level in the portal vein and maximal binding capacity (B max ) and Kd of CCK R in the gallbladder were measured in the four groups by RIA and RBA, and the concentrations of cholesterol in bile were also observed Results Compared with the control group, after high cholesterol feeding for two weeks, the gallbladder emptying rate[(65 83±7 32)%～(47 22±5 24)%] and B max of CCK R[(60±27)～(32±13)fmol/mg protein] and in decreased fasting gallbladder volume(FV) [(0 89±0 26)～(1 34±0 61) cm 3] and concentration of cholesterol[(0 44±0 11)～(0 60±0 13) mmol/L] in bile in creased, but no change was in the serum CCK level and Kd of CCK R in the cholesterol group Compared with the natural group, after two week in take of herb decoction of qingre lidan and liqi huoxue, FV[(1 27±0 60)～(0 90±0 27) cm 3],RV[(0 85±0 45)～(0 32±0 12) cm 3],FB[(0 92±0 35)～(0 73±0 21) cm 3],RB[(0 76±0 34)～(0 29±0 08) cm 3] in the treated group decreased significantly; but gallbladder emptying rate[(43 06±4 27)%～(67 01±6 82)%] increased significantly The concentration of cholesterol in bile was lower in the treated group than in the natural group [(0 59±0 14)～(0 43±0 10) mmol/L], but no change was found in the serum CCK level Bmax of CCK R in the treated group increased significantly[(39±19)～(59±11)fmol/mg protein], Kd of CCK R showed no significant changes between the treated group and natural group. Conclusion High cholesterol in gallblad

关 键 词：胆汁 胆固醇 胆囊收缩素受体 胆囊结石 胆囊收缩功能障碍 

分 类 号：R657.42[医药卫生—外科学]