补阳还五汤对沙土鼠脑缺血再灌注星形胶质细胞的影响  

Effects of Bu Yang Huan Wu Decoction on Astrocytes after Cerebral Ischemia and Reperfusion

作　　者：赖真 王沙燕 耿小茵 邓常青[2] 张阮章 

机构地区：[1]暨南大学医学院附属第二医院深圳市人民医院,广东深圳518020 [2]广州中医药大学糖尿病研究所,广东广州510405

出　　处：《中国中药杂志》2002年第10期763-765,共3页China Journal of Chinese Materia Medica

基　　金：广东省中医药管理局科研项目 (99617)

摘　　要：目的 :探讨补阳还五汤对脑缺血再灌注后星形胶质细胞 (AS)的影响。方法 :采用沙土鼠双侧颈动脉夹闭脑缺血模型 ,于脑缺血 15min、再灌注 2 4h和 4 8h后 ,运用免疫组织化学技术观察星型胶质纤维酸性蛋白(GFAP)的动态表达。结果 :缺血 15min再灌注 2 4h后 ,GFAP免疫阳性反应达高峰 ,补阳还五汤可使GFAP免疫反应减轻 ;缺血 15min再灌注 4 8h后GFAP表达减弱 ,补阳还五汤可使其增强。结论 :补阳还五汤对脑缺血再灌注后星形胶质细胞的调节作用可能与脑缺血损伤后神经功能的恢复有关。Objective: To study the effects of Bu Yang Huan Wu Decoction on astrocytes after cerebral ischemia and reperfusion. Method:Cerebral ischemia model in gerbils was produced by ligating bilateral common carotid artery. The dynamic expressin of GFAP were determined by immunochemistry after cerebyal ischemia for 15min followed by reperfusion for 24 hours and 48 hours. Result: GFAP positive reactions reached a peak after cerebral ischemia for 15 min followed by reperfusion for 24 hours. Bu Yang Huan Wu Decoction decreased the expression. GFAP positive reactions decreased after cerebral ischemia for 15 min followed by reperfusion for 48 hours, Bu Yang Huan Wu Decoction increased the expression. Conclusion:The regulation of Bu Yang Huan Wu Decoction on astrocytes after cerebral ischemia and reperfusion may be related to repairing process after cerebral ischemia.

关 键 词：沙土鼠 缺血再灌注 补阳还五汤 脑缺血 星形胶质细胞 免疫组织化学 

分 类 号：R285.5[医药卫生—中药学]