黄芪、当归对家兔肾缺血再灌注损伤时TNF-α,bFGF的调节作用  

Adjustment Effect of Dadix Astragalus and Dadix Angelicae Sinensis on TNF-α and bFGF on Renal Injury Induced by Ischemia Reperfusion in Rabbit

作　　者：徐晓玉[1] 李莉华[2] 邬丽莎[2] 赵春玲[2] 林海英[2] 

机构地区：[1]重庆医科大学药学系,重庆410061 [2]泸州医学院生理教研室,四川泸州646000

出　　处：《中国中药杂志》2002年第10期771-773,共3页China Journal of Chinese Materia Medica

基　　金：四川省中医药管理局资助项目 (983 3 )

摘　　要：目的 :探讨黄芪、当归对兔肾缺血再灌注 (IR)损伤的防治作用机制。方法 :健康成年日本大耳白兔 34只 ,随机均分至假手术对照 (对照 )组、IR模型组和黄芪 +IR组、当归 +IR组。在肾缺血 1h再灌注 4 8h取肾组织作电镜检查 ,并测血清肌酐 (Cr)、肾组织肿瘤坏死因子 (TNF α)和碱性成纤维细胞生长因子 (bFGF)含量。结果 :IR模型组肾组织变性改变显著 ,黄芪 +IR组、当归 +IR组病变轻微 ;IR模型组血清中Cr、肾组织中TNF α含量较对照组显著增高 ,bFGF显著降低。黄芪 +IR组、当归 +IR组Cr、TNF α含量较IR模型组降低 ,bFGF增高。结论 :黄芪、当归防治肾IR损伤的作用机制可能与其对TNF α和bFGF等细胞因子的调控有关。Objective: To study the mechanism of Astragalus membeanaceus and Angelica sinensis inrenal ischemia reperfusion injury of rabbits. Method: Thirty-four heathy mature Japanese big-ear rabbits were randomized into control group,IR model group, Astragalus Membeanaceus+IR groupand Danggui+IR group.Renal ischemia lasted for 1 hour and then the renal reperfusion lasted for 48 hours.In the end the kidneys were observed with Electron Microscopy and the level of Cr inserum and the level of TNF-αand bFGF in kidneys were measured. Result:The changes of kidneys in IR model group were significant, but they were slight in Astragalus Membeanaceus+IR group and Danggui+IR group.The level of Cr in serum and the level of TNF-α in kidneys of IR model group were higher and yet the level of bFGF decreased obviously.The level of Cr and TNF-αin Astragalus Membeanaceus+IR group and Danggui+IR group was lower than that in IR model group and the level of bFGF was higher than that in IR model group. Conclusion: The mechanism of Astragalus Membeanaceus and Danggui in remedying renal IR injury may be relative to their regulation of TNF-α,bFGF and other cytokines. [

关 键 词：黄芪 当归 家兔 肾缺血 肿瘤坏死因子 碱性成纤维细胞生长因子 缺血再灌 

分 类 号：R285.5[医药卫生—中药学]