电镜观察鸭乙型肝炎病毒在北京鸭肝内的形态发生和繁殖动态  

Ultrastructural Study and Localization of Duck Hepatitis B Virus in the Livers of experimentally Infected Ducks

作　　者：田佩玉 陈鸿珊[1] 黄静[1] 杨文刚[1] 李壮[1] 

机构地区：[1]中国医学科学院医药生物技术研究所

出　　处：《中国医学科学院学报》1991年第2期108-111,I007,8,共6页Acta Academiae Medicinae Sinicae

摘　　要：鸭乙型肝炎病毒(DHBV)静脉感染1日龄北京鸭,9h后鸭肝提取物DHBV-DNA阳性,54h后肝细胞内质网池内可见到病毒颗粒,第4日外周血DHBV-DNA阳性。电镜负染色检查鸭阳性血清,可见DHBV直径40～50nm球形完整病毒和直径40～80nm大小不等类球形表面抗原两种形态,未见丝状和管状体。感染3月后鸭血清持续DHBV-DNA阳性,肝组织超薄切片可见到类似人乙型肝炎病毒(HBV)三种形态:核心颗粒、空心表面抗原和完整病毒颗粒。与人不同的是核心颗粒仅少量存在于核内,大量存在于鸭肝细胞浆内。电镜结果提示DHBV在细胞内增殖可能存在二种途径:(1)病毒的合成和装配似在细胞浆内质网内进行。(2)核心颗粒在核内复制,通过细胞核膜到胞浆,芽生进入内质网,并获得来源于内质网膜的外衣而成熟,类似HBV的繁殖方式。An electron microscopic study was performed to investigate the occurrence and localization of duck hepatitis B virus (DHBV) in the livers of infected Beijing ducks.DHBV-DNA was found in trace amounts in liver extract on 9th hours after infection by Southern blot hybridization and was found in serum on the 4th day of infection.Virus particles were recognized in the hepatocytes as early as the 3rd day of infection. Many incomplet virus particles, (50 to 80 am in diameter), and a few complete virus partides, (45-65 nm in diameter), were seen in cisternae of rough and smooth endoplasmic reticula of hepatocytes, clusters or scatterd core particles, (35-38 nm in diameter) , were seen in the cytoplasm,some core particles were found in nuclei of liver cells where core assembly might be occur.The similarities and differences of the localizations of DHBV and HBV in duck liver cells are discussed.

关 键 词：乙肝病毒 鸭 电子显微镜 观察 

分 类 号：R373.21[医药卫生—病原生物学]