高毒效杀甜菜夜蛾苏云金芽孢杆菌WY-190  

Characterization of Bacillus thuringiensis WY-190 Showing High Performance in Killing Spodoptera exigua

作　　者：牛桂兰[1] 闫建平[1] 郑大胜[1] 袁志明[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国生物防治》2002年第4期166-170,共5页Chinese Journal of Biological Control

基　　金：中国科学院生命科学与生物技术重点项目 (970 316 )

摘　　要：从本实验室分离得到的 4 0 6株苏云金芽孢杆菌中 ,以甜菜夜蛾为供试昆虫 ,采用生物测定的方法 ,筛选出一株对甜菜夜蛾高效的WY 1 90菌株 ,确定其发酵原液LC5 0 为 0 .2 5μl/ml。对WY 1 90的形态特征、生理生化特性和伴孢晶体蛋白进行了观察和研究。研究结果表明 ,WY 1 90属于H3a3b血清型库斯塔克亚种 ;具有几丁质酶活性 ,伴孢晶体形态主要为大小不一的菱形、小正方形、椭圆形 ,并具镶嵌结构 ;SDS PAGE电泳图谱表明主要由 1 35kD和 6 5kD两种蛋白成分组成 ,在菌株生长形态、发酵培养特征、生理生化特性方面与生产菌株HD 1的差别不大 ,其主要差异表现在对甜菜夜蛾的敏感性和杀虫毒力的差别上。A highly effective strain of Bacillus thuringiensis, designated as WY 190, was selected from 406 wild strains via bioassay against neonate larva of Spodoptera exigua. Killing test showed that LC 50 of original fermentation fluid of the strain was 0.25μl/ml. Serological identification indicated that the strain belonged to H 3a3b type (subsp. kurstaki). TEM observation demonstrated that the stain produced parasporal crystal different in size and forms (including rhombic, small square and elliptic). SDS PAGE electrophoresis indicated the crystal was consisted of two proteins with MW 135kD and 65kD respectively. The strain did not show any other difference in growth, fermentation requirement, and biochemical properties when comparing with commercial strain HD 1.

关 键 词：苏云金芽孢杆菌 甜菜夜蛾 生物学特性 WY-190菌株 微生物杀虫剂 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治] S482.39[农业科学—植物保护]