蝴蝶兰花梗节间段培养繁殖的初步研究  

The Preliminary Research on the Rapid Propagation of Phalaenopsis by Using the Joint-points of Flower-stems as Explants

作　　者：鲁雪华[1] 郭文杰[1] 徐立晖 林新华 

机构地区：[1]福建省农业科学院生物技术中心,福州350003 [2]福建省罗源县花卉种苗基地,罗源350600

出　　处：《园艺学报》2002年第5期491-492,共2页Acta Horticulturae Sinica

摘　　要：采用蝴蝶兰植株的茎尖、根尖、花梗节、花梗节间切段作外植体诱导类原球茎 ,其中花梗节间切段诱导频率明显高于其它外植体 ,用改良的MS培养基 +6 BA 5～ 7.5mg/L +NAA 0 .5～ 1.0mg/L +椰乳汁 15 %可加速类原球茎的形成。选用水藓作为试管苗的移栽基质有利于提高小苗的成活率。The stem-tip, root-tip, flower-stem-knot and cutting segment of lower-stem-knot of the plant were used as explants to study the rapid propagation method of Phalaenopsis schilleriana by tissue culture. The result indicated that the explants from different parts of the plants were different in protocorm like body induction rate. Among all the explants, the cutting section of flower-stem-knot was fastest in inducing the portocorm like body. The formation rate of protocorm like body could be accelerated by using the improved MS medium supplemented with 6-BA 5-7.5mg/L, NAA 0.5-1.0mg/L+15%CW. It is beneficial to improving the survival rate to take the Sphagnum moss as the growing base of the tube seedlings in transplanting.

关 键 词：兰梗节间段 蝴蝶兰 组织培养 类原球茎 基质 

分 类 号：S682.31[农业科学—观赏园艺]