灵芝孢子油口服乳的免疫作用研究  

Ganoderma lucidum sppores oil oral emulsion enhancing the function of immune system

作　　者：袁诚[1] 罗爱勤[1] 李菁[1] 陈路林[1] 蒋兆健[1] 陈亮[1] 刘培庆[2] 陈健文[2] YUAN Cheng;LUO Ai-qin;LI Jing;CHEN Lu-lin;JIANG Zhao-jian;CHEN Liang;LIU Pei-qing;CHEN Jian-wen(National Engineer Research Center for the Modernization of Extraction and Separation of TCM, Guangzhou 510240, China;Laboratory of pharmacology and toxicology, Schoool of pharmacy, Sun Yat-sen University, Guangzhou 510006, China)

机构地区：[1]广州白云山汉方现代药业有限公司/中药提取分离过程现代化国家工程研究中心,广州510240 [2]中山大学药学院药理与毒理学实验室,广州510006

出　　处：《肿瘤代谢与营养电子杂志》2016年第2期104-107,共4页Electronic Journal of Metabolism and Nutrition of Cancer

基　　金：广州市科技惠民专项对口科技帮扶专题(2014Y2-00539)

摘　　要：目的研究灵芝孢子油口服乳的免疫增强作用。方法以H22荷瘤小鼠为研究对象,分对照组(玉米油)、阳性对照组(康莱特软胶囊)、灵芝孢子油低组、灵芝孢子油高组、灵芝孢子油口服乳低组、灵芝孢子油口服乳高组6组,观察灵芝孢子油口服乳对荷瘤小鼠的体液免疫功能、网状内皮系统吞噬功能、迟发型变态反应及抑瘤效果和生物调节因子的影响。结果灵芝孢子油口服乳1.2g/kg可增加H22荷瘤小鼠的血清HC50值,可明显增强H22荷瘤小鼠的廓清指数,可适当提高H22荷瘤小鼠的肝脏系数、脾脏系数、吞噬系数;对二硝基氟苯诱导的小鼠迟发型变态反应呈阳性,可显著升高H22荷瘤小鼠血清IL-1、TNF-α、IFN-γ的含量。结论灵芝孢子油口服乳具有显著的增强免疫作用,可提高H22荷瘤小鼠的生物调节因子IL-1、TNF-α、IFN-γ的水平。Objective To study the effects of G. lucidum spore oil oral emulsion an enhancing the function of immune system.Methods H22 Tumor bearing mice as the research object were divided into six groups: negative control group (corn oil), positivecontrol group (Kanglaite Soft Capsules), G. lucidum spore oil low dose group, G. lucidum spore oil high dose group, G. lucidumspore oil oral emulsion low dose group, G. lucidum spore oil oral emulsion high dose group. Observe the effect of G. lucidumspore oil oral emulsion on the function of humoral immunity, phagocytic function of reticuloendothelial system, delayed typeallergic reaction and the effect of inhibiting tumor and biological regulation factors in H22 tumor bearing mice. Results G. lucidumspore oil oral emulsion 1.2g/kg could increase the serum HC50 value of H22 tumor bearing mice, could significantly enhance theclearance index of H22 tumor bearing mice, could improve the liver coefficient, spleen coefficient and phagocytic coefficient ofH22 tumor bearing mice, was positive on DNFB induced delayed hypersensitivity reaction and could significantly increase thecontent of serum IL-1, TNF-alpha, IFN-gamma of H22 tumor bearing mice. Conclusions G. lucidum spore oil oral emulsion canenhance the immune function and improve the levels of biological regulatory factors IL-1, TNF-alpha, IFN-gamma of H22 tumorbearing mice.

关 键 词：灵芝孢子油 口服乳 免疫 

分 类 号：R285.5[医药卫生—中药学]