饲料氧化鱼油对草鱼肝胰脏结构和功能的损伤  

DAMAGE OF OXIDIZED FISH OIL ON THE STRUCTURE AND FUNCTION OF HEPATOPANCREAS OF GRASS CARP(CTENOPHARYNGODON IDELLUS)

作　　者：陈科全[1] 叶元土[1] 蔡春芳[1] 吴萍[1] 黄雨薇[1] 吴韬[1] 林秀秀[1] 罗其刚 张宝彤 萧培珍[1,2] CHEN Ke-Quan;YE Yuan-Tu;CAI Chun-Fang;WU Ping;HUANG Yu-Wei;WU Tao;LIN Xiu-Xiu;LUO Qi-Gang;ZHANG Bao-Tong;XIAO Pei-Zhen(Preclinical Medicine and Biological Science College of Soochow University, Key Laboratory of Aquatic Animal Nutrition in Jiangsu Province, Suzhou 215123, China;Laboratory of Aquatic Animal Nutrition Research System, Beijing Institute of Nutrition,Beijing 100000, China)

机构地区：[1]苏州大学基础医学与生物科学学院,江苏省水产动物营养重点实验室,苏州215123 [2]北京营养源研究所、水产动物系统营养研究开放实验室,北京100000

出　　处：《水生生物学报》2016年第4期793-803,共11页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金项目(31172417);苏州市应用基础研究项目(SYN201316)资助~~

摘　　要：为了探讨饲料氧化鱼油对草鱼(Ctenopharyngodon idellus)肝胰脏组织结构及其功能的影响,研究以豆油、鱼油及氧化鱼油作为饲料脂肪源,分别设计鱼油组(6F)、豆油组(6S)、2%氧化鱼油(4S2OF)、4%氧化鱼油(2S4OF)及6%氧化鱼油(6OF)5组等氮、等能的半纯化饲料,在池塘网箱中养殖72d。结果显示:氧化鱼油显著增加草鱼血清ALB、GLB、MDA和GSH含量(P<0.05),显著降低肝胰脏GSH和SOD含量(P<0.05);氧化鱼油会显著增加草鱼肝胰脏指数及肝胰脏脂肪含量(P<0.05),且草鱼血清TG含量显著上升(P<0.05),HDL/LDL显著下降(P<0.05);氧化鱼油使血清及肝胰脏TC含量显著增加(P<0.05),血清TBA显著下降(P<0.05),肝胰脏TBA显著上升(P<0.05);氧化鱼油会引起草鱼脂肪肝,损伤肝胰脏细胞线粒体,并导致肝胰脏细胞纤维化和组织萎缩。结果表明:饲料添加氧化鱼油会引起草鱼氧化应激,并降低草鱼肝胰脏抗氧化能力;扰乱草鱼肝胰脏脂肪代谢,引起脂肪肝;影响胆汁酸肝肠循环,使胆汁酸在肝胰脏中堆积,并损伤肝胰脏细胞线粒体,最终增加草鱼肝胰脏脂肪性肝炎发生率。To investigate effects of oxidized fish oil on the structure and permeability of hepatopancreas of grass carp(Ctenopharyngodon idellus), five iso-nitrogen and iso-energy diets were formulated with soybean oil, fish oil and oxidizedfish oil at different concentrations, 6% fish oil, 6% soybean oil, 2% oxidized fish oil and 4% soybean oil, 4% oxidizedfish oil and 2% soybean oil, 6% oxidized fish oil for a 72d experiment. Result showed that oxidized fish oil significantlyincreased (P<0.05) the content of ALB, GLB, MDA, GSH of grass carp serum and significantly reduced (P<0.05) the content of GSH, SOD of grass carp hepatopancreas. Oxidized fish oil significantly increased (P<0.05) theHSI, content of lipid of hepatopancreas and TG content of serum, and significantly reduced (P<0.05) the ratio of HDLand LDL at the same time. Oxidized fish oil significantly enhanced the content of TC on serum and hepatopancreas andthe content of TBA on hepatopancreas (P<0.05) but significantly diminished the content of serum TBA (P<0.05). Oxidizedfish oil caused fatty liver by disrupting the fat metabolism, mitochondrial damage of hepatopancreas by impactingbile acid accumulation, hepatopancreas cell fibrosis, and tissue atrophyin grass carp. In conclusion, products of oxidizedfish oil may cause grass carp oxidative stress and decrease antioxidant capacity of grass carp hepatopancreas.

关 键 词：脂肪代谢 胆汁酸 线粒体 草鱼 

分 类 号：S963.1[农业科学—水产养殖]