金鱼草中央细胞中线粒体DNA拷贝数的定量  

Quantification of Mitochondrial DNA Copies in Central Cells of Antirrhium majus

作　　者：曾紫风 孙欢[1] 郭雪[1] 高龙[1] 王丹阳[1] ZENG Zi-feng;SUN Huan;GUO Xue;GAO Long;WANG Dan-yang(Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi’an 710069, China)

机构地区：[1]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,西安710069

出　　处：《热带亚热带植物学报》2016年第4期452-455,共4页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金青年项目(31200234);陕西省教育厅省级重点实验室科研计划项目(14JS089)资助~~

摘　　要：为探究金鱼草(Antirrhinum majus)中央细胞的线粒体DNA拷贝数,采用竞争型定量PCR技术进行了测定。结果表明,金鱼草中央细胞的体积为(61570±732)μm^3,平均携带(783±25)个拷贝的线粒体DNA,并且中央细胞与卵细胞具有相似的体积/线粒体DNA拷贝数比值。推测金鱼草中央细胞包含如此高丰度线粒体DNA可能是为早期胚乳细胞的发育而储备的。The mitochondrial DNA (mtDNA) copies of Antirrhinum majus were measured by quantitative PCRmethod. The results showed that the mean volume of central cell of A. majus was (61570±732) μm3 with (783±25)mtDNA copies. There were similar ratio of volume to mtDNA copy between central cell and egg cell. It waspresumed that the abundant mtDNA in central cell might reserve for the early development of endosperm cells.

关 键 词：中央细胞 线粒体DNA 定量PCR 金鱼草 

分 类 号：Q943.2[生物学—植物学]