入侵植物猫爪藤体外细胞毒活性成分的筛选分离  

Screening and Isolation of Cytotoxicity in vitro from Invasive Plant Macfadyena unguis-cati L.(Bignoniaceae)

作　　者：郑志忠[1,2] 谌迪[2] 刘韶松[2] 邓远[1] 林志灿[2] 明艳林[1,2] ZHENG Zhi-zhong;CHEN Di;LIU Shao-song;DENG Yuan;LIN Zhi-can;MING Yan-lin(College of Chemical Engineering, Huaqiao University, Xiamen 361021, Fujian, China;Xiamen Key Laboratory for Plant Introduction Qarantine and Natural Production, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, Fujian, China)

机构地区：[1]华侨大学化工学院,福建厦门361021 [2]厦门华侨亚热带植物引种园厦门市植物引种检疫与植物源产物重点实验室,福建厦门361002

出　　处：《热带亚热带植物学报》2016年第4期464-470,共7页Journal of Tropical and Subtropical Botany

基　　金：厦门市科技创新基金项目(3502Z20142007)资助~~

摘　　要：为了筛选分离入侵植物猫爪藤的细胞毒活性成分,采用MTT法以75%乙醇提取物的不同组分分别处理人肝癌细胞SMMC7721、Bel7402和正常肝细胞Chang Liver,对他们的体外增殖抑制率进行了研究。结果表明,总醇提物的氯仿组分对肝癌细胞表现出明显的体外增殖抑制作用,其次是石油醚组分。从氯仿萃取组分中分离出具有更强细胞毒活性的成分熊果酸。因此,入侵植物猫爪藤具有体外细胞毒活性,熊果酸是其体外细胞毒活性的主要成分之一。In order to screen and isolate the active constituents from invasive plant Macfadyena unguis-cati L.(Bignoniaceae), the anti-proliferative effects of SMMC7721, Bel7402 by different solvent extraction fractions ofethanol extracts of M. unguis-cati in vitro were studied by using MTT assay. The results showed that thechloroform fraction of ethanol extracts had strong inhibitory effect on liver cancer cells, and then was petroleumether fraction. An active constituent isolated from chloroform fraction, ursolic acid, had strong inhibitory effect.Therefore, invasive plant M. unguis-cati L. has cytotoxicity in vitro, and one of the main active components isursolic acid.

关 键 词：入侵植物 猫爪藤 细胞毒 有效成分 筛选分离 

分 类 号：R284.2[医药卫生—中药学]