枇杷属野生植物种胚离体培养和植株再生研究  

Studies on in vitro Culture and Regeneration of Embryos in Wild Plants of Eriobotrya

作　　者：刘义存[1,2] 黄天启 林顺权[2] LIU Yi-cun;HUANG Tian-qi;LIN Shun-quan(College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225,Guangdong China;College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong China)

机构地区：[1]仲恺农业工程学院园艺园林学院,广东广州510225 [2]华南农业大学园艺学院,广东广州510642

出　　处：《亚热带植物科学》2016年第2期112-116,共5页Subtropical Plant Science

基　　金：广东省科技厅科技基础条件建设项目(2014A030304057;2015A030303015);广州市科技创新委资助华南农业大学(亚热带农业生物资源保护与利用)国家重点实验室项目(201504010028)

摘　　要：以枇杷属Eriobotrya植物11个野生种、1个远缘杂交后代材料为试材,采用胚为外植体,在不同植物生长调节剂配比培养条件下,进行胚离体培养和植株再生研究。结果表明,胚培养的最佳培养基配方为MS+6-BA 1.0 mg·L^(-1)+KT 1.0 mg·L^(-1)+NAA 0.1 mg·L^(-1)。在此培养基中,所有试验材料均获得成功,其中栎叶枇杷E.prinoides的萌发率和丛芽率均达100%,有效地建立了枇杷属野生种的胚离体培养及其植株再生体系。The effects of the combination of the plant growth regulators and culture condition on thein vitro culture and regeneration of the embryo of Eriobotrya plants were studied. The results showedthat the best media in vitro was MS + 6-BA 1.0 mg·L-1 + KT 1.0 mg·L-1 + NAA 0.1 mg·L-1 forembryo culture of 11 wild species and 1 intergeneric hybrid of Eriobotrya plants. All of the explantswere succeed in regeneration from embryo culture, among them, the germination rate and sproutingshoot rate of E. prinoides both were 100%. This experiment had established an effective in vitroculture and plantlet regeneration system of embryos in Eriobotrya.

关 键 词：枇杷属植物 野生种 胚培养 植株再生 

分 类 号：S667.3[农业科学—果树学]