西瓜果实AGPL1基因启动子的序列分析  

Cloning and Sequence Analysis of AGPL1 Promoters in Watermelon Fruit Tissue

作　　者：周丽英[1] 卜璐璐[1] 杨春雷[1] 高婷[1] 田甜 杨正安[1] ZHOU Li-ying;BU Lu-lu;YANG Chun-lei;GAO Ting;TIAN Tian;YANG Zheng-an

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]巧家县农技推广中心,云南巧家654600

出　　处：《亚热带植物科学》2016年第2期122-126,共5页Subtropical Plant Science

基　　金：国家自然科学基金项目(31260481;31460516);云南省自然科学基金项目(2011FB049)

摘　　要：利用PCR技术从西瓜嫩叶中扩增出1.2 kb AGPL1基因启动子,通过Plant CARE和PLACE数据库对启动子序列进行生物信息学分析。结果表明,AGPL1启动子具有多个典型的TATA-Box和CAAT-Box等基本元件,以及高水平转录顺式作用元件5UTR Py-rich stretch,光响应元件ACE、ATCC-motif、Box 4、I-Box、Sp1、TCCC-motif、GAG-motif、MNF1,2个赤霉素响应元件GARE-motif、P-box,参与蛋白代谢调节的顺式作用元件O2-site,参与水杨酸反应的顺式作用元件TCA-element,厌氧诱导所需的顺式作用元件ARE、逆境胁迫响应元件TC-rich repeats、生理昼夜节律控制元件circadian等,为研究外源基因在果实中的特异表达提供参考。To make use of plant fruit specific express foreign proteins, 1.2 kb AGPL1 gene promoterwas amplified in watermelon leaves using PCR, and bioinformatics analysis of AGPL1 promotersequences was conducted. The results showed by PlantCARE database and PLACE database thatAGPL1 gene promoter had number of typical core elements such as TATA-Box, CAAT-Box, highlevel transcription cis-acting element 5UTR Py-rich stretch, photoresponse elements including ACE,ATCC-motif, Box 4, I-Box, Sp1, TCCC-motif, GAG-motif, MNF1, two gibberellin responseelements GARE-motif and P-box, cis-acting element related to protein metabolic regulation O2-site,cis-acting element related to salicylic acid reaction TCA-element, induced anaerocabically cis-actingelement ARE, element involved in defense and stress responsiveness TC-rich repeats, circadianrhythm controlling element circadian and so on, all of which lays the groundwork for such researchon regulation of PG genes in the future.

关 键 词：西瓜 AGPL1 启动子 

分 类 号：Q943.2[生物学—植物学]