铜纳米簇催化鲁米诺化学发光体系的研究及应用  

Enhanced chemiluminescence of the luminol-hydrogen peroxide system by copper nanoclusters and its analytical application

作　　者：吴秀明[1] 胡欣[1] 王光丽[1] WU Xiu-ming;HU Xin;WANG Guang-li*(The Key Laboratory of Food Colloids and Biotechnology,Ministry of Education,School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品胶体与生物技术教育部重点实验室,化学与材料工程学院,江苏无锡214122

出　　处：《化学研究与应用》2016年第8期1070-1074,共5页Chemical Research and Application

基　　金：国家自然科学基金项目(21275065,21575052)资助

摘　　要：合成了牛血清白蛋白修饰的铜纳米簇(BSA-Cu NCs),研究发现在碱性环境中,BSA-Cu NCs 对鲁米诺-过氧化氢体系的发光信号有很好的增强作用。对铜纳米簇催化鲁米诺-过氧化氢化学发光的机理进行了研究,并发现色氨酸对鲁米诺-过氧化氢-铜纳米簇化学发光体系信号具有增强作用,基于此,建立了化学发光测定色氨酸的含量的方法。该方法对色氨酸的检测限为6×10-8 mol·L-1 ,线性范围为2． 0 ×10-7 ~10-4 mol·L-1 。Bovine serum albumin modified Cu nanoclusters(BSA-Cu NCs)were synthesized,and it was found that Cu NCs could enhancethe chemiluminescence(CL)emission of the luminol – H2 O2 system in an alkaline medium. The CL spectra and radical scavengerswere conducted to investigate the possible CL enhancement mechanism. Interestingly,tryptophan was found to enhance theCL emission of Cu NCs – luminol – H2 O2 system greatly,based on which,a novel CL assay for the detection of tryptophan was developedwith a linear range of 2. 0 × 10-7 – 10-4 mol·L-1 and a detection limit as low as 6×10-8 mol·L-1 .

关 键 词：化学发光 鲁米诺 铜纳米簇 色氨酸 

分 类 号：O657.3[理学—分析化学]