一测多评法同时测定山楂叶中6种有效成分的含量  

Simultaneous Determination of Six Effective Components in Crataegus pinnatifida by Quantitative Analysis of Multi-components by Single Marker

作　　者：杨明宇[1] 高婧 杜义龙[1] 李艳荣[1] 赵胜男[1] 潘海峰[1] YANG Mingyu;GAO Jing;DU Yilong;LI Yanrong;ZHAO Shengnan;PAN Haifeng(Key Laboratory of Study and Development of Traditional Chinese Medicine in Hebei Province,Chengde Medical University,Hebei Chengde 067000,China;Chengde Center for Food and Drug Control,Hebei Chengde 067000,China)

机构地区：[1]承德医学院河北省中药研究与开发重点实验室,河北承德067000 [2]承德市食品药品检验检测中心,河北承德067000

出　　处：《中国药房》2016年第24期3404-3407,共4页China Pharmacy

基　　金：河北省高等学校科学技术研究项目(No.ZD2015097);河北省高等学校科学研究计划项目(No.冀教科〔2015〕7号)

摘　　要：目的：建立同时测定山楂叶中绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷含量的方法。方法：采用高效液相色谱法，以牡荆素葡萄糖苷为基准峰，分别计算其与绿原酸、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的相对校正因子（RCF），通过RCF计算山楂叶中上述5种成分的含量。色谱柱为AgilentZORBAXSBC18，流动相为0.1％甲酸-乙腈-四氢呋喃（梯度洗脱），流速为1.0ml/min，检测波长为350nm，柱温为30℃，进样量为10μl。结果：绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷检测进样量线性范围分别为12.50～400.0μg（r＝0.9998）、25.00～800.0μg（r＝0.9999）、31.25～1000.0μg（r＝0.9999）、6.470～260.0μg（r＝0.9999）、2.50～80.0μg（r＝0.9998）、9.375～300.0μg（r＝0.9999）；精密度、稳定性、重复性试验的RSD＜2.0％；加样回收率分别为99.2％～103.9％（RSD＝1.6％）、97.9％～100.8％（RSD＝1.2％）、99.2％～100.8％（RSD＝0.5％）、97.3％～101.3％（RSD＝1.5％）、98.0％～103.0％（RSD＝1.9％）、95.5％～101.5％（RSD＝2.2％），n均为6。牡荆素葡萄糖苷与绿原酸、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷间的RCF分别为1.119、1.009、0.706、1.063、0.830。结论：该方法操作简便，精密度、稳定性、重复性好，可用于6种有效成分含量的同时测定。OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid,vitexin glucoside,vitexinrhamnoside,vitexin,rutin and hyperoside in Crataegus pinnatifida. METHODS:With reference peak of vitexin glucoside,HPLC was conducted to calculate the relative correction factor(RCF)of chlorogenic acid,vitexin glucoside,vitexin rhamnoside,vitexin,rutin and hyperoside,then the contents of above-mentioned 5 components in C. pinnatifida were calculated. The columnwas Agilent ZORBAX SB C18 with mobile phase of 0.1% formic acid-acetonitrile-tetrahydrofuran(gradient elution)at a flow rateof 1.0 ml/min,the detection wavelength was 350 nm,column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 12.50-400.0 μg for chlorogenic acid(r=0.999 8),25.00-800.0 μg for vitexin glucoside(r=0.999 9),31.25-1 000.0 μg for vitexin rhamnoside(r=0.999 9),6.470-260.0 μg for vitexin(r=0.999 9),2.50-80.0 μg for rutin(r=0.999 8)and 9.375-300.0 μg for hyperoside(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoverieswere 99.2%-103.9%(RSD=1.6%,n=6),97.9%-100.8%(RSD=1.2%,n=6),99.2%-100.8%(RSD=0.5%,n=6),97.3%-101.3%(RSD=1.5%,n=6),98.0%-103.0%(RSD=1.9%,n=6)and 95.5%-101.5%(RSD=2.2%,n=6). RCFs of vitexinglucoside with chlorogenic acid,vitexin rhamnoside,vitexin,rutin and hyperoside were 1.119,1.009,0.706,1.063 and 0.830,respectively. CONCLUSIONS:The method is simple with good precision,stability and reproducibility,and it can be sued for thesimultaneous determination of 6 components in C. pinnatifida.

关 键 词：山楂叶 一测多评法 相对校正因子 质量控制 

分 类 号：R284.1[医药卫生—中药学] R927[医药卫生—中医学]