重组短链脱氢酶LcSDR催化(R)-1-苯基乙醇不对称合成的工艺开发  

The Process Development of(R)-1-Phenylethanol Asymmetric Reduction Catalyzed by A Recombinant Short-Chain Dehydrogenase and Reductase LcSDR

作　　者：陈敏[1] 王亚军[1] 柳志强[1] 罗希[1] 郑裕国[1] Chen Min;Wang Yajun;Liu Zhiqiang;Luo Xi;Zheng Yuguo(Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China)

机构地区：[1]浙江工业大学生物工程学院浙江省生物有机合成技术研究重点实验室,浙江杭州310014

出　　处：《化学反应工程与工艺》2016年第4期320-325,372,共7页Chemical Reaction Engineering and Technology

基　　金：国家自然科学基金(21476209)

摘　　要：采用基因挖掘技术从Lactobacillus composti基因组中筛选到一条编码短链脱氢酶(LcSDR)的序列,该LcSDR能不对称还原苯乙酮(1a)合成(R)-1-苯基乙醇[(R)-1b]。构建了Lc SDR和葡萄糖脱氢酶(EsGDH)双酶偶联催化合成体系,优化了Lc SDR不对称还原1a合成(R)-1b的催化工艺参数。在较佳催化条件下,50 g/L1a转化反应2 h,产物(R)-1b得率93.8%,产物对映体过量值(eep)高于99%,该手性生物合成催化过程的时空产率达到562.8 g/(L·d)。A short-chain dehydrogenases and reductases (LcSDR) coding sequence lcsdr was screened outfrom Lactobacillus composti genome via gene mining technology. It was found that LcSDR was able toasymmetrically reduce acetophenone(1a) to (R)-1-phenylethanol [(R)-1b]. The LcSDR and glucosedehydrogenase (EsGDH) double-enzyme conjugate catalytic synthesis system was constructed, and theprocess parameters were optimized for catalytic reduction of 1a to (R)-1b in this study. Under the appropriateconditions, 50 g/L 1a was converted to (R)-1b in 2 h with yield of 93.8%, the enantiomeric excess (eep)above 99% and the space time yield of 562.8 g/(L·d).

关 键 词：短链脱氢酶 (R)-1-苯基乙醇 不对称还原 辅酶再生 

分 类 号：O621.34[理学—有机化学] Q814.9[理学—化学]