星点设计-效应面法优化半枝莲总生物碱提取工艺  

Optimization of Extraction Process of Scutellaria barbata Total Alkaloids with Central-Composite-Design Response-Surface Method

作　　者：刘欣[1] 杨新[1] 张丽[1] 方振峰[1] 张涛[1] LIU Xin;YANG Xin;ZHANG Li;FANG Zhenfeng;ZHANG Tao(Department of Pharmacy,School of Medicine,Jianghan University,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学医学院药学系,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2016年第5期448-455,共8页Journal of Jianghan University：Natural Science Edition

基　　金：江汉大学研究生科研创新基金(301004210001)

摘　　要：目的采用星点设计-效应面法优化半枝莲总生物碱回流提取工艺。方法采用紫外分光光度法检测半枝莲总生物碱含量,对乙醇浓度、提取时间、固液比、提取温度、提取次数5个因素进行考察,确定对半枝莲总生物碱提取量影响最大的因素并确定其取值范围,在此基础上进行星点设计实验,SAS软件建立预测模型,采用效应面法优化最佳提取条件。结果确定最优提取工艺条件为温度85℃,40.06倍量55.20%乙醇回流提取2次,每次113.29 min。最佳工艺条件下,测得总生物碱提取量为26.932 9 mg/g,实测值与预测值偏差小于2%,二项式拟合的相关系数R2=0.997 5。结论采用星点设计-效应面法优化半枝莲总生物碱提取工艺,方法简便,工艺稳定,预测性良好。Objective To optimize the extraction process of Scutellariabar bata total alkaloids with central- composite- design response- surface method. Methods Five main factors including ethanol volume fraction,extraction time,solid-liquid ratio,extraction temperature,and extraction times on this reflux extraction process were investigated,to screen the influence of various factors on the yield of S. barbata total alkaloid extraction with ultraviolet spectrophotometry. Central composite design was used to design experiments,and SAS was used to establish a prediction model,and response surface method was used to abtain the best extraction process. Results The optimal process conditions were 40. 06 times of 55. 20%ethanol reflux extraction under 85 ℃ for two times,each 113. 29 min. Under the optimal process conditions,the total alkaloid content was 26. 932 9 mg/g,and the deviation of predicted values and measured values were less than 2%. The binomial fitting correlation coefficient was R2 =0. 997 5. Conclusion Central-composite-design response-surface methods is successfully used for the optimization on theextraction process of S. barbatatotal alkaloids,with good predictability and stability.

关 键 词：半枝莲 总生物碱 提取工艺 星点设计 效应面法 

分 类 号：R284.2[医药卫生—中药学]