杨树环化酶基因组织表达模式和蛋白定位  

Gene Expression Pattern and Subcellular Localization of Populus Trichocarpa Cyclase

作　　者：贾志刚[1] 夏德安[1] 李淑娟[1] JIA Zhi-gang;XIA De-an;LI Shu-juan(State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, PRC)

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040

出　　处：《湖南农业科学》2016年第10期1-3,共3页Hunan Agricultural Sciences

基　　金：国家"863"计划课题(2013AA102702)

摘　　要：半定量RT-PCR显示,毛果杨形成层、幼叶和顶芽中的Potri.006G237100转录产物极低,但在木质部、叶柄和根中其转录水平却较髙,在木质化茎节其转录产物也呈现高丰度积累,这表明Potri.006G237100在毛果杨木质化组织中特异地、高丰度转录表达。实验构建p GWB5-Potri.006G237100载体,转化拟南芥、分子鉴定得到5个过量表达转基因植株,激光共聚焦分析显示融合蛋白Potri.006G237100-GFP定位在转基因植株的细胞质。Semi-quantitative RT-PCR analysis showed that Potri.006G237100 gene transcripts are very low in cambium, young leaf andapical bud of Populus trichocarpa, but high in xylem, petiole and root. Also, high abundance of Potri.006G237100 gene transcripts wasdetected in lignifying stems. These data suggest that Potri.006G237100 gene is specifically and abundantly expressed in the lignifyingtissues of P. trichocarpa. In addition, the DNA fragement of Potri.006G237100 gene was constructed into plant expression vector pGWB5and transformed into Arabidopsis plant. Five transgenic plants were attained through molecular identification. Confocal laser scanningmicroscope analysis suggested that Potri.006G237100-GFP fusion protein is located in the cytoplasm of transgenic plants.

关 键 词：环化酶 组织表达模式 Potri.006G237100 蛋白定位 杨树 

分 类 号：S792.11[农业科学—林木遗传育种]