核桃青皮提取物对烟草病毒的生长抑制作用  

Growth Inhibition Effect of Extracts from Walnut Green Husk on Tobacco Virus

作　　者：何念武[1] 黄金辉 张永锋 穆耀辉 HE Nian-wu;HUANG Jin-hui;ZHANG Yong-feng;MU Yao-hui(College of Biology Pharmacy and Food Engineering,Shangluo University,Shangluo 726000,China;Shangluo Municipal Tobacco Company in Shaanxi Province,Shangluo 726000,China)

机构地区：[1]商洛学院生物医药与食品工程学院,商洛726000 [2]陕西省烟草公司商洛市公司,商洛726000

出　　处：《价值工程》2016年第34期209-211,共3页Value Engineering

基　　金：陕西省教育厅专项科研计划项目:商洛核桃青皮提取物防治烟草病虫害关键技术研究(编号:15JK1219);商洛市烟草公司横向科研项目:核桃青皮提取物防治烟草病毒病关键技术研究(编号:KJ-2014-02)

摘　　要：[目的]探究核桃青皮不同溶剂提取物对烟草病毒的生长抑制效果。[方法]以商洛本地所产核桃青皮为原料,分别采用乙醚、氯仿、乙醇、乙酸乙酯和丙酮五种溶剂制备提取物。采用回流提取法制备提取物,再用半叶法测定不同溶剂提取物对烟草花叶病毒和烟草黄瓜花叶病毒的生长抑制作用。[结果]在试验条件下核桃青皮不同溶剂提取物对烟草花叶病毒和烟草黄瓜花叶病毒均有一定的生长抑制作用,并且伴随着提取物浓度的不断增加呈现出良好的剂量效应关系。其中乙酸乙酯提取物效果最好,抑制率最高分别可达到65.9%、52.3%,与同浓度的阳性对照几乎无明显差异。[结论]核桃青皮乙酸乙酯提取物对烟草病毒具有良好的防治效果。[Objective] To explore the different solvent extracts from walnut green husk on tobacco virus growth inhibitory effect. [Methods] Taking Shangluo local production of walnut green husk as raw material, using ethyl ether, chloroform, ethanol, ethyl acetate and acetone five solvent extracts were prepared by refluxing method. The extract was prepared by reflux extraction method, and then the growth inhibition effect of different solvent extracts on tobacco mosaic virus and cucumber mosaic virus was determined by half leaf method. [Results] In the test conditions of different solvent extracts from walnut green husk on tobacco mosaic virus and tobacco cucumber mosaic virus has certain inhibitory effect, and with the increasing concentration of the extracts showed a good dose-response relationship. The ethyl acetate extract effect best, the highest inhibition rate could reach 65.9%, 52.3%, almost no the obvious difference with the positive control of the same concentration. [Conclusion] The ethyl acetate extract of walnut green husk has good control effect on tobacco virus.

关 键 词：核桃青皮 提取物 烟草花叶病毒 烟草黄瓜花叶病毒 生长抑制 

分 类 号：S314[农业科学—作物栽培与耕作技术]