铁皮石斛蒴果的离体保存  

Research on Conservation of Dendrobium Candidum Capsule in Vitro

作　　者：王辉[1] 罗云[1] 李凤[1] 吴饶 齐泽民[1] W ANGHui;LUO Yun;LI Feng;WU Rao;Ql Ze-min

机构地区：[1]内江师范学院生命科学学院,四川内江641199

出　　处：《内江师范学院学报》2016年第6期34-36,共3页Journal of Neijiang Normal University

基　　金：四川省教育厅重点项目(15ZA0287;14ZA0250);四川省教育厅高校科研创新团队项目(14TD0025)

摘　　要：以铁皮石斛杂交蒴果为材料,研究在不同保存条件下蒴果内种子的萌发率和萌发后周长变化情况,目的是筛选出铁皮石斛蒴果离体保存的适宜条件.结果表明:4℃冰箱保存30、60、150d的蒴果情况较好;种子接种于1/2MS+NAA0.2mg/L+土豆汁150g/L培养基上萌发较好,萌发率平均为81.77%,萌发10d的种子周长较其他处理大,可作为铁皮石斛蒴果采收后短期保存的方法.Using the hybrid capsule of Dendrobiumcandidumas the material,the germination and perimeter of its seeds were studied in different preservation conditions in order to find out about the suitable conditions for vitro conservation.The result showed that the optimal conservation condition is when it is kept for 30 d,60 d and 150 d under the temperature of 4℃in refrigerator.The seeds is found to have an average germination rate of 81.77% when it is planted in the substrate of 1/2MS+ NAA0.2 mg/L+ potato juice 150 g/L.After 10 of germination,the perimeter is larger,compared with the effect by other treating methods.It proves that such a method can be used as a method for short-term preservation of Dendrobium Candidum Capsule in Vitro after harvest.

关 键 词：铁皮石斛蒴果 离体保存 萌发率 周长 

分 类 号：Q943.1[生物学—植物学]