超声/碱预处理对青霉素菌渣厌氧消化的影响研究  

Effect study of ultrasonic/alkaline pretreatment on anaerobic digestion of penicillin bacterial residue

作　　者：李再兴[1,2] 高妍[1,2] 钟为章[1,2] 李贵霞[1,2] 刘春[1,2] 郭嘉运 王勇军 杨永会 LI Zaixing;GAO Yan;ZHONG Weizhang;LI Guixia;LIU Chun;GUO Jiayun;WANG Yongjun;YANG Yonghui(School of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018,China;Pollution Prevention Biotechnology Laboratory of Hebei Province, Shijiazhuang, Hebei 050018,China;Hengshui No. 1 High School, Hengshui, Hebei 053000,China;Environmental Protection Institute,NCPC,Shijiazhuang, Hebei 050015, China)

机构地区：[1]河北科技大学环境科学与工程学院,河北石家庄050018 [2]河北省污染防治生物技术实验室,河北石家庄050018 [3]河北衡水第一中学,河北衡水053000 [4]华北制药集团有限责任公司环境保护研究所,河北石家庄050015

出　　处：《河北科技大学学报》2016年第3期302-308,共7页Journal of Hebei University of Science and Technology

基　　金：国家自然科学基金(51478160);河北省重大技术创新项目(12276703Z)

摘　　要：为了提高菌渣厌氧消化生产沼气的效率,最终实现菌渣的资源化和减量化,采用超声/碱预处理方法处理青霉素菌渣,考察了pH值、超声声能密度、含水率和反应时间对预处理效果的影响,并通过生化产甲烷潜力(BMP)试验考察了菌渣的可生化性。正交试验结果表明:超声/碱预处理可强化菌渣破壁效果,促进胞内有机物溶出,其最佳预处理条件:pH值为10,声能密度为2.0 W/mL,含水率为97%,反应时间为5 min,COD溶出率最高能达到84.69%,是单独超声预处理的2.08倍。BMP试验表明,预处理各因素对菌渣沼气产率的影响程度为含水率>声能密度>反应时间>pH值。按照甲烷产率确定最佳预处理条件:pH值为9,声能密度为0.5 W/mL,含水率为96%,反应时间为30min,其甲烷产率最高可达335mL/g,是未处理菌渣甲烷产率的2.2倍。In order to improve the efficiency of anaerobic digestion for biogas production and achieve the resource recovery and decrement of antibiotic bacterial residue,ultrasonic/alkaline pretreatment is used to treat penicillin bacterial residues(PBR),the influence of pH value,ultrasonic sound energy density,moisture content and reaction time on pretreatment effect are inves-tigated,and the biodegradability of PBR is evaluated by biochemical methane potential(BMP)test.The orthogonal experiment result shows that ultrasonic/alkaline pretreatment can enhance the residue cell wall broken effect,and promote intracellular organic matter dissolution.The optimum pretreatment conditions are pH of 10,ultrasonic sound energy density of 2.0W/mL,moisture content of 97%,and pretreatment time of 5min.In this case,the highest COD dissolution rate can reach 84.69%,which is 2.08 times higher than that by the ultrasonic pretreatment only.The BMP test results show that the impact of the pretreatment factors is as follows:moisture content > ultrasonic sound energy density > reaction time > pH value.The optimum pretreatment conditions according to methane productivity are pH of 9,ultrasonic sound energy density of 0.5 W/mL,moisture content of 96%,and reaction time of 30 min.In this case,the methane yield rate can reached 335mL/g,which is 2.2times higher than that from the untreated bacteria residue.

关 键 词：三废处理与综合利用 青霉素菌渣 超声/碱预处理 厌氧消化 生化产甲烷潜力 

分 类 号：X703[环境科学与工程—环境工程]