强的松增强HBV感染小鼠体内病毒复制表达  

Prednisone enhancing viral replication and expression in HBV transfection mice

作　　者：于毅[1,2] 刘凤君[1] 石瑶[1,3] 陆明娇 魏小丽[1] YU Yi;LIU Feng-jun;SHI Ya0;LU Ming-jiao;WEI Xiao-li(Department o f Infection, Affiliated Hospital o f North Sichuan Medical College ,Nanchong 637000;Department o f Infection,Dazhou People's Hospital ,Dazhou 635000;Internal M edicine, The Third People's Hospital o f Chengdu, Chengdu 610000 , Sichuan, China)

机构地区：[1]川北医学院附属医院感染科,四川南充637000 [2]达州市人民医院感染科,四川达州635000 [3]成都市第三人民医院内科,四川成都610000

出　　处：《川北医学院学报》2016年第3期331-335,共5页Journal of North Sichuan Medical College

基　　金：四川省卫生厅项目(100150)

摘　　要：目的：检测糖皮质激素对HBV感染小鼠体内HBV复制的作用。方法：采用高压注射体内转染法将HBV复制型重组质粒pAAV/HBV1.2导人雄性C57BL/6小鼠，转染后24h按体重、年龄、血清HBeAg水平对小鼠进行配对，分为实验组和对照组，分别连续给予强的松或生理盐水灌胃。于第3天和第10天给药后4~6h处死小鼠。采用Southern杂交检测小鼠肝脏HBV-DNA复制中间体;化学发光法检测血清中HBeAg和HBsAg。结果：对HBV感染小鼠连续给予强的松或生理盐水3d后，所有小鼠肝脏中均可检测到较高水平的HBV-DNA复制中间体，给予强的松的实验组稍高于给予生理盐水的对照组，二者比值为1.127:1;连续处理10d后，实验组小鼠肝脏中HBV-DNA复制中间体的水平明显高于对照组，比值为2.534:1。处理3d后对照组和实验组小鼠血清HBsAg平均值分别为：1618.28666.23叫/^L，实验组与对照组无明显差另1|;小鼠血清对照组和实验组HBeAg平均值分别为14.68网/PLJ4.95实验组对照组无明显差别；处理10d后对照组和实验组小鼠血清HBsAg平均值分别为：8059.4〇网/^LaiSS5.87实验组高于对照组。小鼠血清对照组和实验组1?(^8平均值分别为183.15(^8/(^、286.14(^8/(^，实验组高于对照组。结论：强的松可增强111^感染小鼠体内乙肝病毒的复制和表达，并且随着作用时间的延长，作用也越明显。本研究为糖皮质激素及引起HBV再激活的相关研究奠定基础，同时也再次提醒临床医生，应充分重视糖皮质激素的使用中HBV再激活的问题。Objective: To detect the effect of glucorticoid on HBV replication of mice in vivo. Methods: HBV replication competentplasmid pAAV/HBVl. 2 was transferred into male C57L/6 mice by hydrodynamics-based in vivo transfection. 24 h after transfection,the mice were matched by body weight, age and serum level of Hepatitis B e antigen ( HBeAg) and were divided into experimentgroup and control group, and then treated orally with prednisone or normal saline at interval of twenty four hours for 3 and 10 days. Fourto six hours after the last administration, the mice were killed. HBV-DNA replication intermediates in liver were analyzed by SouthernBlot Hybridization. The serum HBsAg and HBeAg were detected by chemiluminescent detection system. Results : HBV-DNA replicationintermediates were detected in the livers of mice after treating orally with prednisone or normal saline for 3 days, the level of HBV-DNAreplication intermediates of the experimental group was 0. 127 times higher than that of the control group. However,after treatment for 10days, compared with the control group of m ice, the level of HBV-DNA replication intermediates of the experimental mice increasedgreatly. Quantitative analysis showed that prednisone enhanced the level of HBV-DNA replication intermediates appropriately 1. 534fold. Three days after the above treatment, the serum HBsAg of the control mice and the experimental mice were 1 618. 28 (jLg/(jiL and1 666. 23 (jLg/(jiL on average respectively, which showed no significant difference. Similarly, there was no significant difference in the serumHBeAg,which showed as follows: 14. 68 (jig/(jlL , 14. 95 (jig/(jlL on average respectively. In contrast, 10 days after the same treatment, the serum HBsAg of the control and the experimental of mice were on average 8 059. 40 (jig/(jlL , 11 885. 87 (jig/(jlL , respectively,and the serum HBeAg were 183. 15 (jLg/(jiL, 286. 14 (jLg/(jiL, respectively. Both of the level of serum HBsAg and HbeAg from experi-mental mice were higher than that of control animals. Conclusi

关 键 词：强的松 乙型肝炎病毒 复制 小鼠模型 高压注射体内转染法 

分 类 号：R512.6[医药卫生—内科学]